Raman microscopy for cellular investigations – from single cell imaging to drug carrier uptake visualization

Birthe Kann, Herman L. Offerhaus, Maike Windbergs, Cornelis Otto

Research output: Contribution to journalArticleAcademicpeer-review

77 Citations (Scopus)

Abstract

Progress in advanced therapeutic concepts requires the development of appropriate carrier systems for intracellular drug delivery. Consequently, analysis of interaction between carriers, drugs and cells as well as their uptake and intracellular fate is a current focus of research interest. In this context, Raman spectroscopy recently became an emerging analytical technique, due to its non-destructive, chemically selective and label-free working principle. In this review, we briefly present the state-of-the-art technologies for cell visualization and drug internalization. Against this background, Raman microscopy is introduced as a versatile analytical technique. An overview of various Raman spectroscopy investigations in this field is given including interactions of cells with drug molecules, carrier systems and other nanomaterials. Further, Raman instrumentations and sample preparation methods are discussed. Finally, as the analytical limit is not reached yet, a future perspective for Raman microscopy in pharmaceutical and biomedical research on the single cell level is given
Original languageEnglish
Pages (from-to)71-90
JournalAdvanced drug delivery reviews
Volume89
DOIs
Publication statusPublished - 2015

Keywords

  • METIS-313475
  • IR-99834

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