Rapid Method for Analyzing Gadolinium Binding Activity using Colorimetric Assay Employing PAR

Meri Ayurini; Tony Ekkelenkamp; Jos Paulusse; J.F.J. Engbersen

doi:10.15408/jkv.v5i1.9238

Rapid Method for Analyzing Gadolinium Binding Activity using Colorimetric Assay Employing PAR

Meri Ayurini^*, Tony Ekkelenkamp, Jos Paulusse, J.F.J. Engbersen

^*Corresponding author for this work

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Abstract

In this research, we study a colorimetric assay employing 4-(2-pyridylazo)-resorcinol (PAR) for quantifying the chelated and free gadolinium in the system. This is a preliminary study in order to observe the complexation behavior between Gd³⁺ and targeted molecules. This was carried out in 100 mM MES buffer at pH 5.5. The binding constant (Log K) of the Gd³⁺-PAR (1:1) complex at pH 5.5 is 4.41 ± 0.09 M^-1 . Since this method only uses UV-Vis spectrometry, it is concluded that colorimetric assay employing PAR is a fast and inexpensive method in order to analyze the binding activity between Gd³⁺ and targeted ligands.

Original language	English
Pages (from-to)	23-34
Number of pages	12
Journal	Jurnal Kimia Valensi
Volume	5
Issue number	1
DOIs	https://doi.org/10.15408/jkv.v5i1.9238
Publication status	Published - May 2019

Keywords

Colorimetric assay
Gadolinium
MRI contrast agent

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10.15408/jkv.v5i1.9238Licence: CC BY-SA

ArticleFinal published version, 1.03 MBLicence: CC BY-SA

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Rapid Method for Analyzing Gadolinium Binding Activity using Colorimetric Assay Employing PAR

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