Abstract
| Original language | Undefined |
|---|---|
| Pages (from-to) | 054119 |
| Number of pages | 11 |
| Journal | Biomicrofluidics |
| Volume | 8 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Sep 2014 |
Keywords
- EWI-25471
- IR-93312
- METIS-309756
Cite this
}
Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis. / De, Arpita; Sparreboom, Wouter; van den Berg, Albert; Carlen, Edwin.
In: Biomicrofluidics, Vol. 8, No. 5, 09.2014, p. 054119.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis
AU - De, Arpita
AU - Sparreboom, Wouter
AU - van den Berg, Albert
AU - Carlen, Edwin
N1 - eemcs-eprint-25471
PY - 2014/9
Y1 - 2014/9
N2 - Genetic sequence and hyper-methylation profile information from the promoter regions of tumor suppressor genes are important for cancer disease investigation. Since hyper-methylated DNA (hm-DNA) is typically present in ultra-low concentrations in biological samples, such as stool, urine, and saliva, sample enrichment and amplification is typically required before detection. We present a rapid microfluidic solid phase extraction (mu SPE) system for the capture and elution of low concentrations of hm-DNA (<= 1 ng ml(-1)), based on a protein-DNA capture surface, into small volumes using a passive microfluidic lab-on-a-chip platform. All assay steps have been qualitatively characterized using a real-time surface plasmon resonance (SPR) biosensor, and quantitatively characterized using fluorescence spectroscopy. The hm-DNA capture/elution process requires less than 5 min with an efficiency of 71% using a 25 mu l elution volume and 92% efficiency using a 100 mu l elution volume. (C) 2014 AIP Publishing LLC.
AB - Genetic sequence and hyper-methylation profile information from the promoter regions of tumor suppressor genes are important for cancer disease investigation. Since hyper-methylated DNA (hm-DNA) is typically present in ultra-low concentrations in biological samples, such as stool, urine, and saliva, sample enrichment and amplification is typically required before detection. We present a rapid microfluidic solid phase extraction (mu SPE) system for the capture and elution of low concentrations of hm-DNA (<= 1 ng ml(-1)), based on a protein-DNA capture surface, into small volumes using a passive microfluidic lab-on-a-chip platform. All assay steps have been qualitatively characterized using a real-time surface plasmon resonance (SPR) biosensor, and quantitatively characterized using fluorescence spectroscopy. The hm-DNA capture/elution process requires less than 5 min with an efficiency of 71% using a 25 mu l elution volume and 92% efficiency using a 100 mu l elution volume. (C) 2014 AIP Publishing LLC.
KW - EWI-25471
KW - IR-93312
KW - METIS-309756
U2 - 10.1063/1.4899059
DO - 10.1063/1.4899059
M3 - Article
VL - 8
SP - 054119
JO - Biomicrofluidics
JF - Biomicrofluidics
SN - 1932-1058
IS - 5
ER -