Three different, complementary soft lithographic approaches for the fabrication of chemical patterns on ultrathin polystyrene-block-poly(tert-butyl acrylate) (PS690-b-PtBA1210) films are discussed. Central to the methodology is the previously introduced reactive PS690-b-PtBA1210 platform that allows one to covalently graft (bio)molecules via robust amide linkages in high densities on flat, as well as on structured, surfaces. As shown in this paper, the combination of the polymer-based platform and reactive microcontact printing (μCP) patterning approaches allows one to obtain patterns of (bio)molecules with (sub)micrometer feature sizes. The μCP approaches comprise: (A) the direct transfer of functional (bio)molecules from an oxidized elastomeric stamp to hydrolyzed and N-hydroxysuccinimide (NHS) activated PS690-b-PtBA1210; (B) the transfer of a passivating poly(ethylene glycol) layer to hydrolyzed and NHS-activated PS690-b-PtBA1210 followed by wet chemical grafting of functional moieties; (C) the local hydrolysis of the PtBA skin layer with trifluoroacetic acid (TFA), followed by NHS activation and wet chemical derivatization. The applicability and the versatility of the combination of the polymer thin film-based platform and soft lithographic methodologies for patterning biologically relevant molecules is demonstrated for polyamidoamine (PAMAM) dendrimers, different proteins, as well as probe DNA. The successful hybridization of target DNA and the immobilization of fibronectin in micropatterns show that ultrahigh density patterns for micro- and nano-arrays, as well as for studies of cell–surface interactions, can be conveniently fabricated based on these approaches and platforms.