Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

Arpita De; Jan William van Nieuwkasteele; Edwin Carlen; Albert van den Berg

doi:10.1109/Transducers.2013.6627386

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

Arpita De, Jan William van Nieuwkasteele, Edwin Carlen, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

3 Citations (Scopus)

5 Downloads (Pure)

Abstract

Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement

Original language	Undefined
Title of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII
Place of Publication	Barcelona
Publisher	IEEE
Pages	2795-2798
Number of pages	4
ISBN (Print)	978-1-4673-5983-2
DOIs	https://doi.org/10.1109/Transducers.2013.6627386
Publication status	Published - 16 Jun 2013
Event	17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013 - Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013 Conference number: 17 http://www.transducers-eurosensors2013.org/

Publication series

Name	TRANSDUCERS & EUROSENSORS XXVII
Publisher	IEEE
Volume	2013

Conference

Conference	17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013
Abbreviated title	TRANSDUCERS 2013
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13
Other	Held in conjunction with EUROSENSORS XXVII
Internet address	http://www.transducers-eurosensors2013.org/

Keywords

EWI-24119
METIS-300227
IR-88285

Access to Document

10.1109/Transducers.2013.6627386

http://eprints.eemcs.utwente.nl/secure2/24119/01/06627386.pdf

Cite this

De, A., van Nieuwkasteele, J. W., Carlen, E., & van den Berg, A. (2013). Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. In The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII (pp. 2795-2798). (TRANSDUCERS & EUROSENSORS XXVII; Vol. 2013). IEEE. https://doi.org/10.1109/Transducers.2013.6627386

@inproceedings{fdfe5ede48b943de9c118af02b1a5661,

title = "Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors",

abstract = "Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement",

keywords = "EWI-24119, METIS-300227, IR-88285",

author = "Arpita De and {van Nieuwkasteele}, {Jan William} and Edwin Carlen and {van den Berg}, Albert",

note = "10.1109/Transducers.2013.6627386 ; 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013 ; Conference date: 16-06-2013 Through 20-06-2013",

year = "2013",

month = jun,

day = "16",

doi = "10.1109/Transducers.2013.6627386",

language = "Undefined",

isbn = "978-1-4673-5983-2",

series = "TRANSDUCERS & EUROSENSORS XXVII",

publisher = "IEEE",

pages = "2795--2798",

booktitle = "The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII",

address = "United States",

url = "http://www.transducers-eurosensors2013.org/",

}

De, A, van Nieuwkasteele, JW, Carlen, E & van den Berg, A 2013, Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. in The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII. TRANSDUCERS & EUROSENSORS XXVII, vol. 2013, IEEE, Barcelona, pp. 2795-2798, 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6627386

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. / De, Arpita; van Nieuwkasteele, Jan William; Carlen, Edwin et al.
The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII. Barcelona: IEEE, 2013. p. 2795-2798 (TRANSDUCERS & EUROSENSORS XXVII; Vol. 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

AU - De, Arpita

AU - van Nieuwkasteele, Jan William

AU - Carlen, Edwin

AU - van den Berg, Albert

N1 - Conference code: 17

PY - 2013/6/16

Y1 - 2013/6/16

N2 - Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement

AB - Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement

KW - EWI-24119

KW - METIS-300227

KW - IR-88285

U2 - 10.1109/Transducers.2013.6627386

DO - 10.1109/Transducers.2013.6627386

M3 - Conference contribution

SN - 978-1-4673-5983-2

T3 - TRANSDUCERS & EUROSENSORS XXVII

SP - 2795

EP - 2798

BT - The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII

PB - IEEE

CY - Barcelona

T2 - 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013

Y2 - 16 June 2013 through 20 June 2013

ER -

De A, van Nieuwkasteele JW, Carlen E, van den Berg A. Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. In The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII. Barcelona: IEEE. 2013. p. 2795-2798. (TRANSDUCERS & EUROSENSORS XXVII). doi: 10.1109/Transducers.2013.6627386