Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

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    Abstract

    Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement
    Original languageUndefined
    Title of host publicationThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII
    Place of PublicationBarcelona
    PublisherIEEE
    Pages2795-2798
    Number of pages4
    ISBN (Print)978-1-4673-5983-2
    DOIs
    Publication statusPublished - 16 Jun 2013
    Event17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013 - Barcelona, Spain
    Duration: 16 Jun 201320 Jun 2013
    Conference number: 17
    http://www.transducers-eurosensors2013.org/

    Publication series

    NameTRANSDUCERS & EUROSENSORS XXVII
    PublisherIEEE
    Volume2013

    Conference

    Conference17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013
    Abbreviated titleTRANSDUCERS 2013
    CountrySpain
    CityBarcelona
    Period16/06/1320/06/13
    OtherHeld in conjunction with EUROSENSORS XXVII
    Internet address

    Keywords

    • EWI-24119
    • METIS-300227
    • IR-88285

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