Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

Arpita De; Jan William van Nieuwkasteele; Edwin Carlen; Albert van den Berg

doi:10.1109/Transducers.2013.6627386

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

3 Citations (Scopus)

5 Downloads (Pure)

Abstract

Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement

Original language	Undefined
Title of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII
Place of Publication	Barcelona
Publisher	IEEE
Pages	2795-2798
Number of pages	4
ISBN (Print)	978-1-4673-5983-2
DOIs	https://doi.org/10.1109/Transducers.2013.6627386
Publication status	Published - 16 Jun 2013
Event	17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013 - Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013 Conference number: 17 http://www.transducers-eurosensors2013.org/

Publication series

Name	TRANSDUCERS & EUROSENSORS XXVII
Publisher	IEEE
Volume	2013

Conference

Conference	17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013
Abbreviated title	TRANSDUCERS 2013
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13
Other	Held in conjunction with EUROSENSORS XXVII
Internet address	http://www.transducers-eurosensors2013.org/

Keywords

EWI-24119
METIS-300227
IR-88285

Access to Document

10.1109/Transducers.2013.6627386

http://eprints.eemcs.utwente.nl/secure2/24119/01/06627386.pdf

Cite this

De, A., van Nieuwkasteele, J. W., Carlen, E., & van den Berg, A. (2013). Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. In The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII (pp. 2795-2798). (TRANSDUCERS & EUROSENSORS XXVII; Vol. 2013). IEEE. https://doi.org/10.1109/Transducers.2013.6627386

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title = "Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors",

abstract = "Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be \textasciitilde{}1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement",

keywords = "EWI-24119, METIS-300227, IR-88285",

author = "Arpita De and \{van Nieuwkasteele\}, \{Jan William\} and Edwin Carlen and \{van den Berg\}, Albert",

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doi = "10.1109/Transducers.2013.6627386",

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series = "TRANSDUCERS \& EUROSENSORS XXVII",

publisher = "IEEE",

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De, A, van Nieuwkasteele, JW, Carlen, E & van den Berg, A 2013, Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. in The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII. TRANSDUCERS & EUROSENSORS XXVII, vol. 2013, IEEE, Barcelona, pp. 2795-2798, 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2013, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6627386

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. / De, Arpita; van Nieuwkasteele, Jan William; Carlen, Edwin et al.
The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII. Barcelona: IEEE, 2013. p. 2795-2798 (TRANSDUCERS & EUROSENSORS XXVII; Vol. 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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AU - van Nieuwkasteele, Jan William

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N1 - Conference code: 17

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N2 - Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement

AB - Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement

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De A, van Nieuwkasteele JW, Carlen E, van den Berg A. Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. In The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers & Eurosensors XXVII. Barcelona: IEEE. 2013. p. 2795-2798. (TRANSDUCERS & EUROSENSORS XXVII). doi: 10.1109/Transducers.2013.6627386