TY - JOUR
T1 - Real-Time Monitoring of Nanoparticle Formation by FRET Imaging
AU - Sanchez-Gaytan, Brenda L.
AU - Fay, Francois
AU - Hak, Sjoerd
AU - Alaarg, Amr Muhmed Sabry Abdelhakeem
AU - Fayad, Zahi A.
AU - Perez-Medina, Carlos
AU - Mulder, Willem J.M.
AU - Zhao, Yiming
PY - 2017/1/23
Y1 - 2017/1/23
N2 - Understanding the formation process of nanoparticles is of the utmost importance to improve their design and production. This especially holds true for self-assembled nanoparticles whose formation processes have been largely overlooked. Herein, we present a new technology that integrates a microfluidic-based nanoparticle synthesis method and Förster resonance energy transfer (FRET) microscopy imaging to visualize nanoparticle self-assembly in real time. Applied to different nanoparticle systems, for example, nanoemulsions, drug-loaded block-copolymer micelles, and nanocrystal-core reconstituted high-density lipoproteins, we have shown the approach's unique ability to investigate key parameters affecting nanoparticle formation.
AB - Understanding the formation process of nanoparticles is of the utmost importance to improve their design and production. This especially holds true for self-assembled nanoparticles whose formation processes have been largely overlooked. Herein, we present a new technology that integrates a microfluidic-based nanoparticle synthesis method and Förster resonance energy transfer (FRET) microscopy imaging to visualize nanoparticle self-assembly in real time. Applied to different nanoparticle systems, for example, nanoemulsions, drug-loaded block-copolymer micelles, and nanocrystal-core reconstituted high-density lipoproteins, we have shown the approach's unique ability to investigate key parameters affecting nanoparticle formation.
KW - METIS-321795
KW - IR-103742
U2 - 10.1002/anie.201611288
DO - 10.1002/anie.201611288
M3 - Article
SN - 0044-8249
VL - 56
SP - 2923
EP - 2926
JO - Angewandte Chemie
JF - Angewandte Chemie
IS - 11
ER -