Recent developments in optical detection methods for microchip separations

S. Götz, U. Karst

    Research output: Contribution to journalArticleAcademicpeer-review

    150 Citations (Scopus)

    Abstract

    This paper summarizes the features and performances of optical detection systems currently applied in order to monitor separations on microchip devices. Fluorescence detection, which delivers very high sensitivity and selectivity, is still the most widely applied method of detection. Instruments utilizing laser-induced fluorescence (LIF) and lamp-based fluorescence along with recent applications of light-emitting diodes (LED) as excitation sources are also covered in this paper. Since chemiluminescence detection can be achieved using extremely simple devices which no longer require light sources and optical components for focusing and collimation, interesting approaches based on this technique are presented, too. Although UV/vis absorbance is a detection method that is commonly used in standard desktop electrophoresis and liquid chromatography instruments, it has not yet reached the same level of popularity for microchip applications. Current applications of UV/vis absorbance detection to microchip separations and innovative approaches that increase sensitivity are described. This article, which contains 85 references, focuses on developments and applications published within the last three years, points out exciting new approaches, and provides future perspectives on this field.
    Original languageUndefined
    Pages (from-to)183-192
    JournalAnalytical and bioanalytical chemistry
    Volume387
    Issue number1
    DOIs
    Publication statusPublished - 2007

    Keywords

    • IR-72266
    • Optical detection methods - Microchip - Microfluidic - Separation - Review
    • METIS-246539

    Cite this

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    title = "Recent developments in optical detection methods for microchip separations",
    abstract = "This paper summarizes the features and performances of optical detection systems currently applied in order to monitor separations on microchip devices. Fluorescence detection, which delivers very high sensitivity and selectivity, is still the most widely applied method of detection. Instruments utilizing laser-induced fluorescence (LIF) and lamp-based fluorescence along with recent applications of light-emitting diodes (LED) as excitation sources are also covered in this paper. Since chemiluminescence detection can be achieved using extremely simple devices which no longer require light sources and optical components for focusing and collimation, interesting approaches based on this technique are presented, too. Although UV/vis absorbance is a detection method that is commonly used in standard desktop electrophoresis and liquid chromatography instruments, it has not yet reached the same level of popularity for microchip applications. Current applications of UV/vis absorbance detection to microchip separations and innovative approaches that increase sensitivity are described. This article, which contains 85 references, focuses on developments and applications published within the last three years, points out exciting new approaches, and provides future perspectives on this field.",
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    author = "S. G{\"o}tz and U. Karst",
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    language = "Undefined",
    volume = "387",
    pages = "183--192",
    journal = "Analytical and bioanalytical chemistry",
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    Recent developments in optical detection methods for microchip separations. / Götz, S.; Karst, U.

    In: Analytical and bioanalytical chemistry, Vol. 387, No. 1, 2007, p. 183-192.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Recent developments in optical detection methods for microchip separations

    AU - Götz, S.

    AU - Karst, U.

    PY - 2007

    Y1 - 2007

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    AB - This paper summarizes the features and performances of optical detection systems currently applied in order to monitor separations on microchip devices. Fluorescence detection, which delivers very high sensitivity and selectivity, is still the most widely applied method of detection. Instruments utilizing laser-induced fluorescence (LIF) and lamp-based fluorescence along with recent applications of light-emitting diodes (LED) as excitation sources are also covered in this paper. Since chemiluminescence detection can be achieved using extremely simple devices which no longer require light sources and optical components for focusing and collimation, interesting approaches based on this technique are presented, too. Although UV/vis absorbance is a detection method that is commonly used in standard desktop electrophoresis and liquid chromatography instruments, it has not yet reached the same level of popularity for microchip applications. Current applications of UV/vis absorbance detection to microchip separations and innovative approaches that increase sensitivity are described. This article, which contains 85 references, focuses on developments and applications published within the last three years, points out exciting new approaches, and provides future perspectives on this field.

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