Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

4 Citations (Scopus)
4 Downloads (Pure)

Abstract

Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.
Original languageEnglish
Title of host publicationProtein Cages
Subtitle of host publicationMethods in Molecular Biology (Methods and Protocols)
EditorsBrendan P. Orner
Place of PublicationNew York
PublisherHumana Press
Pages61-67
Number of pages7
Volume1252
ISBN (Electronic)978-1-4939-2131-7
ISBN (Print)978-1-4939-2130-0
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Number1252
Volume1252

Fingerprint

Bacterial Proteins
Virion
Thermotoga maritima
Brevibacterium
Viruses
Virus Assembly
Ultracentrifugation
Organelles
Sucrose
Proteins
cesium chloride

Keywords

  • METIS-308591
  • IR-95116
  • Bacterial encapsulins
  • Nanotechnology
  • Protein cages
  • Virus purification
  • Virus-like assemblies

Cite this

Rurup, W. F., Cornelissen, J. J. L. M., & Koay, M. S. T. (2015). Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages. In B. P. Orner (Ed.), Protein Cages: Methods in Molecular Biology (Methods and Protocols) (Vol. 1252, pp. 61-67). (Methods in Molecular Biology; Vol. 1252, No. 1252). New York: Humana Press. https://doi.org/10.1007/978-1-4939-2131-7_6
Rurup, W.F. ; Cornelissen, Jeroen Johannes Lambertus Maria ; Koay, M.S.T. / Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages. Protein Cages: Methods in Molecular Biology (Methods and Protocols). editor / Brendan P. Orner. Vol. 1252 New York : Humana Press, 2015. pp. 61-67 (Methods in Molecular Biology; 1252).
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Rurup, WF, Cornelissen, JJLM & Koay, MST 2015, Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages. in BP Orner (ed.), Protein Cages: Methods in Molecular Biology (Methods and Protocols). vol. 1252, Methods in Molecular Biology, no. 1252, vol. 1252, Humana Press, New York, pp. 61-67. https://doi.org/10.1007/978-1-4939-2131-7_6

Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages. / Rurup, W.F.; Cornelissen, Jeroen Johannes Lambertus Maria; Koay, M.S.T.

Protein Cages: Methods in Molecular Biology (Methods and Protocols). ed. / Brendan P. Orner. Vol. 1252 New York : Humana Press, 2015. p. 61-67 (Methods in Molecular Biology; Vol. 1252, No. 1252).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Rurup WF, Cornelissen JJLM, Koay MST. Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages. In Orner BP, editor, Protein Cages: Methods in Molecular Biology (Methods and Protocols). Vol. 1252. New York: Humana Press. 2015. p. 61-67. (Methods in Molecular Biology; 1252). https://doi.org/10.1007/978-1-4939-2131-7_6