Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages

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Abstract

Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.
Original languageEnglish
Title of host publicationProtein Cages
Subtitle of host publicationMethods in Molecular Biology (Methods and Protocols)
EditorsBrendan P. Orner
Place of PublicationNew York
PublisherHumana Press
Pages61-67
Number of pages7
Volume1252
ISBN (Electronic)978-1-4939-2131-7
ISBN (Print)978-1-4939-2130-0
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Number1252
Volume1252

Keywords

  • METIS-308591
  • IR-95116
  • Bacterial encapsulins
  • Nanotechnology
  • Protein cages
  • Virus purification
  • Virus-like assemblies

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