Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages

W.F. Rurup; Jeroen Johannes Lambertus Maria Cornelissen; M.S.T. Koay

doi:10.1007/978-1-4939-2131-7_6

Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages

W.F. Rurup, Jeroen Johannes Lambertus Maria Cornelissen, M.S.T. Koay

Biomolecular Nanotechnology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

4 Citations (Scopus)

17 Downloads (Pure)

Abstract

Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.

Original language	English
Title of host publication	Protein Cages
Subtitle of host publication	Methods in Molecular Biology (Methods and Protocols)
Editors	Brendan P. Orner
Place of Publication	New York
Publisher	Humana Press
Pages	61-67
Number of pages	7
Volume	1252
ISBN (Electronic)	978-1-4939-2131-7
ISBN (Print)	978-1-4939-2130-0
DOIs	https://doi.org/10.1007/978-1-4939-2131-7_6
Publication status	Published - 2015

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Number	1252
Volume	1252

Keywords

METIS-308591
IR-95116
Bacterial encapsulins
Nanotechnology
Protein cages
Virus purification
Virus-like assemblies

Access to Document

10.1007/978-1-4939-2131-7_6

Cite this

Rurup, W. F., Cornelissen, J. J. L. M., & Koay, M. S. T. (2015). Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages. In B. P. Orner (Ed.), Protein Cages: Methods in Molecular Biology (Methods and Protocols) (Vol. 1252, pp. 61-67). (Methods in Molecular Biology; Vol. 1252, No. 1252). Humana Press. https://doi.org/10.1007/978-1-4939-2131-7_6

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abstract = "Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.",

keywords = "METIS-308591, IR-95116, Bacterial encapsulins, Nanotechnology, Protein cages, Virus purification, Virus-like assemblies",

author = "W.F. Rurup and Cornelissen, {Jeroen Johannes Lambertus Maria} and M.S.T. Koay",

year = "2015",

doi = "10.1007/978-1-4939-2131-7_6",

language = "English",

isbn = "978-1-4939-2130-0",

volume = "1252",

series = "Methods in Molecular Biology",

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editor = "Orner, {Brendan P.}",

booktitle = "Protein Cages",

address = "United States",

}

Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages. / Rurup, W.F.; Cornelissen, Jeroen Johannes Lambertus Maria; Koay, M.S.T.
Protein Cages: Methods in Molecular Biology (Methods and Protocols). ed. / Brendan P. Orner. Vol. 1252 New York: Humana Press, 2015. p. 61-67 (Methods in Molecular Biology; Vol. 1252, No. 1252).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

TY - CHAP

T1 - Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages

AU - Rurup, W.F.

AU - Cornelissen, Jeroen Johannes Lambertus Maria

AU - Koay, M.S.T.

PY - 2015

Y1 - 2015

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AB - Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.

KW - METIS-308591

KW - IR-95116

KW - Bacterial encapsulins

KW - Nanotechnology

KW - Protein cages

KW - Virus purification

KW - Virus-like assemblies

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SN - 978-1-4939-2130-0

VL - 1252

T3 - Methods in Molecular Biology

SP - 61

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BT - Protein Cages

A2 - Orner, Brendan P.

PB - Humana Press

CY - New York

ER -

Recombinant expression and purification of 'virus-like' bacterial encapsulin protein cages

Abstract

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Keywords

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