TY - JOUR
T1 - Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
AU - Koch, Lena Sophie
AU - Buentello, David Choy
AU - Broersen, Kerensa
N1 - Funding Information:
This work was supported by the Netherlands Organ-on-Chip Initiative, an NWO Gravitation project (024.003.001) funded by the Ministry of Education, Culture and Science of the government of the Netherlands. D.C.B. thankfully acknowledges the financial support of Consejo Nacional de Ciencia y Tecnología (CONACyT) in the form of a doctoral scholarship.
Publisher Copyright:
© 2023, Journal of Visualized Experiments. All rights reserved.
Financial transaction number:
2500067256
PY - 2023/5/12
Y1 - 2023/5/12
N2 - The currently available animal and cellular models do not fully recapitulate the complexity of changes that take place in the aging human brain. A recent development of procedures describing the generation of human cerebral organoids, derived from human induced pluripotent stem cells (iPSCs), has the potential to fundamentally transform the ability to model and understand the aging of the human brain and related pathogenic processes. Here, an optimized protocol for generating, maintaining, aging, and characterizing human iPSC-derived cerebral organoids is presented. This protocol can be implemented to generate brain organoids in a reproducible manner and serves as a step-by-step guide, incorporating the latest techniques that result in improved organoid maturation and aging in culture. Specific issues related to organoid maturation, necrosis, variability, and batch effects are being addressed. Taken together, these technological advances will allow the modeling of brain aging in organoids derived from a variety of young and aged human donors, as well as individuals afflicted with age-related brain disorders, allowing the identification of physiologic and pathogenic mechanisms of human brain aging.
AB - The currently available animal and cellular models do not fully recapitulate the complexity of changes that take place in the aging human brain. A recent development of procedures describing the generation of human cerebral organoids, derived from human induced pluripotent stem cells (iPSCs), has the potential to fundamentally transform the ability to model and understand the aging of the human brain and related pathogenic processes. Here, an optimized protocol for generating, maintaining, aging, and characterizing human iPSC-derived cerebral organoids is presented. This protocol can be implemented to generate brain organoids in a reproducible manner and serves as a step-by-step guide, incorporating the latest techniques that result in improved organoid maturation and aging in culture. Specific issues related to organoid maturation, necrosis, variability, and batch effects are being addressed. Taken together, these technological advances will allow the modeling of brain aging in organoids derived from a variety of young and aged human donors, as well as individuals afflicted with age-related brain disorders, allowing the identification of physiologic and pathogenic mechanisms of human brain aging.
UR - http://www.scopus.com/inward/record.url?scp=85160475477&partnerID=8YFLogxK
U2 - 10.3791/64586
DO - 10.3791/64586
M3 - Article
C2 - 37246867
AN - SCOPUS:85160475477
SN - 1940-087X
VL - 2023
JO - Journal of visualized experiments
JF - Journal of visualized experiments
IS - 195
M1 - e64586
ER -