Rotational motion and rheotaxis of human sperm do not require functional CatSper channels and transmembrane Ca2+ signaling

Christian Schiffer, Steffen Rieger, Christoph Brenker, Samuel Young, Hussein Hamzeh, Dagmar Wachten, Frank Tüttelmann, Albrecht Röpke, U. Benjamin Kaupp, Alice Wagner, Claudia Krallmann, Sabine Kliesch, Carsten Fallnich*, Timo Strünker

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)
20 Downloads (Pure)

Abstract

Navigation of sperm in fluid flow, called rheotaxis, provides long-range guidance in the mammalian oviduct. The rotation of sperm around their longitudinal axis (rolling) promotes rheotaxis. Whether sperm rolling and rheotaxis require calcium (Ca2+) influx via the sperm-specific Ca2+ channel CatSper, or rather represent passive biomechanical and hydrodynamic processes, has remained controversial. Here, we study the swimming behavior of sperm from healthy donors and from infertile patients that lack functional CatSper channels, using dark-field microscopy, optical tweezers, and microfluidics. We demonstrate that rolling and rheotaxis persist in CatSper-deficient human sperm. Furthermore, human sperm undergo rolling and rheotaxis even when Ca2+ influx is prevented. Finally, we show that rolling and rheotaxis also persist in mouse sperm deficient in both CatSper and flagellar Ca2+-signaling domains. Our results strongly support the concept that passive biomechanical and hydrodynamic processes enable sperm rolling and rheotaxis, rather than calcium signaling mediated by CatSper or other mechanisms controlling transmembrane Ca2+ flux.

Original languageEnglish
Article numbere102363
Number of pages15
JournalEMBO journal
Volume39
Issue number4
DOIs
Publication statusPublished - 17 Feb 2020

Keywords

  • UT-Hybrid-D
  • CatSper
  • human sperm
  • rheotaxis
  • rolling
  • Ca signaling

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