Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 11425-11430 |
| Number of pages | 6 |
| Journal | Langmuir |
| Volume | 25 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - 2009 |
| Externally published | Yes |
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Keywords
- IR-99765
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Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles. / Lindhoud, Saskia; Voorhaar, Lenny; de Vries, Renko; Schweins, Ralf; Cohen Stuart, Martien A.; Norde, Willem.
In: Langmuir, Vol. 25, No. 19, 2009, p. 11425-11430.Research output: Contribution to journal › Article › Academic
TY - JOUR
T1 - Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles
AU - Lindhoud, Saskia
AU - Voorhaar, Lenny
AU - de Vries, Renko
AU - Schweins, Ralf
AU - Cohen Stuart, Martien A.
AU - Norde, Willem
PY - 2009
Y1 - 2009
N2 - The salt-induced disintegration of lysozyme-filled polyelectrolyte complex micelles, consisting of positively charged homopolymers (PDMAEMA150), negatively charged diblock copolymers (PAA42-PAAm417), and lysozyme, has been studied with dynamic light scattering (DLS) and small-angle neutron scattering (SANS). These measurements show that, from 0 to 0.2 M NaCl, both the hydrodynamic radius (Rh) and the core radius (Rcore) decrease with increasing salt concentration. This suggests that the micellar structures rearrange. Moreover, from ∼0.2 to 0.4 M NaCl the light-scattering intensity is constant. In this salt interval, the hydrodynamic radius increases, has a maximum at 0.3 M NaCl, and subsequently decreases. This behavior is observed in both a lysozyme-containing system and a system without lysozyme. The SANS measurements on the lysozyme-filled micelles do not show increased intensity or a larger core radius at 0.3 M NaCl. This indicates that from 0.2 to 0.4 M NaCl another structure is formed, consisting of just the diblock copolymer and the homopolymer, because at 0.12 M NaCl the lysozyme−PAA42-PAAm417 complex has disintegrated. One may expect that the driving force for the formation of the complex in this salt range is other than electrostatic.
AB - The salt-induced disintegration of lysozyme-filled polyelectrolyte complex micelles, consisting of positively charged homopolymers (PDMAEMA150), negatively charged diblock copolymers (PAA42-PAAm417), and lysozyme, has been studied with dynamic light scattering (DLS) and small-angle neutron scattering (SANS). These measurements show that, from 0 to 0.2 M NaCl, both the hydrodynamic radius (Rh) and the core radius (Rcore) decrease with increasing salt concentration. This suggests that the micellar structures rearrange. Moreover, from ∼0.2 to 0.4 M NaCl the light-scattering intensity is constant. In this salt interval, the hydrodynamic radius increases, has a maximum at 0.3 M NaCl, and subsequently decreases. This behavior is observed in both a lysozyme-containing system and a system without lysozyme. The SANS measurements on the lysozyme-filled micelles do not show increased intensity or a larger core radius at 0.3 M NaCl. This indicates that from 0.2 to 0.4 M NaCl another structure is formed, consisting of just the diblock copolymer and the homopolymer, because at 0.12 M NaCl the lysozyme−PAA42-PAAm417 complex has disintegrated. One may expect that the driving force for the formation of the complex in this salt range is other than electrostatic.
KW - IR-99765
U2 - 10.1021/la901591p
DO - 10.1021/la901591p
M3 - Article
VL - 25
SP - 11425
EP - 11430
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 19
ER -