Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles

Saskia Lindhoud, Lenny Voorhaar, Renko de Vries, Ralf Schweins, Martien A. Cohen Stuart, Willem Norde

Research output: Contribution to journalArticleAcademic

35 Citations (Scopus)

Abstract

The salt-induced disintegration of lysozyme-filled polyelectrolyte complex micelles, consisting of positively charged homopolymers (PDMAEMA150), negatively charged diblock copolymers (PAA42-PAAm417), and lysozyme, has been studied with dynamic light scattering (DLS) and small-angle neutron scattering (SANS). These measurements show that, from 0 to 0.2 M NaCl, both the hydrodynamic radius (Rh) and the core radius (Rcore) decrease with increasing salt concentration. This suggests that the micellar structures rearrange. Moreover, from ∼0.2 to 0.4 M NaCl the light-scattering intensity is constant. In this salt interval, the hydrodynamic radius increases, has a maximum at 0.3 M NaCl, and subsequently decreases. This behavior is observed in both a lysozyme-containing system and a system without lysozyme. The SANS measurements on the lysozyme-filled micelles do not show increased intensity or a larger core radius at 0.3 M NaCl. This indicates that from 0.2 to 0.4 M NaCl another structure is formed, consisting of just the diblock copolymer and the homopolymer, because at 0.12 M NaCl the lysozyme−PAA42-PAAm417 complex has disintegrated. One may expect that the driving force for the formation of the complex in this salt range is other than electrostatic.
Original languageEnglish
Pages (from-to)11425-11430
Number of pages6
JournalLangmuir
Volume25
Issue number19
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Disintegration
lysozyme
Micelles
disintegration
Muramidase
Polyelectrolytes
micelles
Enzymes
Salts
salts
radii
Neutron scattering
Homopolymerization
Block copolymers
copolymers
neutron scattering
light scattering
Hydrodynamics
hydrodynamics
Dynamic light scattering

Keywords

  • IR-99765

Cite this

Lindhoud, S., Voorhaar, L., de Vries, R., Schweins, R., Cohen Stuart, M. A., & Norde, W. (2009). Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles. Langmuir, 25(19), 11425-11430. https://doi.org/10.1021/la901591p
Lindhoud, Saskia ; Voorhaar, Lenny ; de Vries, Renko ; Schweins, Ralf ; Cohen Stuart, Martien A. ; Norde, Willem. / Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles. In: Langmuir. 2009 ; Vol. 25, No. 19. pp. 11425-11430.
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abstract = "The salt-induced disintegration of lysozyme-filled polyelectrolyte complex micelles, consisting of positively charged homopolymers (PDMAEMA150), negatively charged diblock copolymers (PAA42-PAAm417), and lysozyme, has been studied with dynamic light scattering (DLS) and small-angle neutron scattering (SANS). These measurements show that, from 0 to 0.2 M NaCl, both the hydrodynamic radius (Rh) and the core radius (Rcore) decrease with increasing salt concentration. This suggests that the micellar structures rearrange. Moreover, from ∼0.2 to 0.4 M NaCl the light-scattering intensity is constant. In this salt interval, the hydrodynamic radius increases, has a maximum at 0.3 M NaCl, and subsequently decreases. This behavior is observed in both a lysozyme-containing system and a system without lysozyme. The SANS measurements on the lysozyme-filled micelles do not show increased intensity or a larger core radius at 0.3 M NaCl. This indicates that from 0.2 to 0.4 M NaCl another structure is formed, consisting of just the diblock copolymer and the homopolymer, because at 0.12 M NaCl the lysozyme−PAA42-PAAm417 complex has disintegrated. One may expect that the driving force for the formation of the complex in this salt range is other than electrostatic.",
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Lindhoud, S, Voorhaar, L, de Vries, R, Schweins, R, Cohen Stuart, MA & Norde, W 2009, 'Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles' Langmuir, vol. 25, no. 19, pp. 11425-11430. https://doi.org/10.1021/la901591p

Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles. / Lindhoud, Saskia; Voorhaar, Lenny; de Vries, Renko; Schweins, Ralf; Cohen Stuart, Martien A.; Norde, Willem.

In: Langmuir, Vol. 25, No. 19, 2009, p. 11425-11430.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles

AU - Lindhoud, Saskia

AU - Voorhaar, Lenny

AU - de Vries, Renko

AU - Schweins, Ralf

AU - Cohen Stuart, Martien A.

AU - Norde, Willem

PY - 2009

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N2 - The salt-induced disintegration of lysozyme-filled polyelectrolyte complex micelles, consisting of positively charged homopolymers (PDMAEMA150), negatively charged diblock copolymers (PAA42-PAAm417), and lysozyme, has been studied with dynamic light scattering (DLS) and small-angle neutron scattering (SANS). These measurements show that, from 0 to 0.2 M NaCl, both the hydrodynamic radius (Rh) and the core radius (Rcore) decrease with increasing salt concentration. This suggests that the micellar structures rearrange. Moreover, from ∼0.2 to 0.4 M NaCl the light-scattering intensity is constant. In this salt interval, the hydrodynamic radius increases, has a maximum at 0.3 M NaCl, and subsequently decreases. This behavior is observed in both a lysozyme-containing system and a system without lysozyme. The SANS measurements on the lysozyme-filled micelles do not show increased intensity or a larger core radius at 0.3 M NaCl. This indicates that from 0.2 to 0.4 M NaCl another structure is formed, consisting of just the diblock copolymer and the homopolymer, because at 0.12 M NaCl the lysozyme−PAA42-PAAm417 complex has disintegrated. One may expect that the driving force for the formation of the complex in this salt range is other than electrostatic.

AB - The salt-induced disintegration of lysozyme-filled polyelectrolyte complex micelles, consisting of positively charged homopolymers (PDMAEMA150), negatively charged diblock copolymers (PAA42-PAAm417), and lysozyme, has been studied with dynamic light scattering (DLS) and small-angle neutron scattering (SANS). These measurements show that, from 0 to 0.2 M NaCl, both the hydrodynamic radius (Rh) and the core radius (Rcore) decrease with increasing salt concentration. This suggests that the micellar structures rearrange. Moreover, from ∼0.2 to 0.4 M NaCl the light-scattering intensity is constant. In this salt interval, the hydrodynamic radius increases, has a maximum at 0.3 M NaCl, and subsequently decreases. This behavior is observed in both a lysozyme-containing system and a system without lysozyme. The SANS measurements on the lysozyme-filled micelles do not show increased intensity or a larger core radius at 0.3 M NaCl. This indicates that from 0.2 to 0.4 M NaCl another structure is formed, consisting of just the diblock copolymer and the homopolymer, because at 0.12 M NaCl the lysozyme−PAA42-PAAm417 complex has disintegrated. One may expect that the driving force for the formation of the complex in this salt range is other than electrostatic.

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JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 19

ER -

Lindhoud S, Voorhaar L, de Vries R, Schweins R, Cohen Stuart MA, Norde W. Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles. Langmuir. 2009;25(19):11425-11430. https://doi.org/10.1021/la901591p