Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging

Mirella J.J. Haartmans, Britt S.R. Claes, Kaj S. Emanuel, Gabrielle J.M. Tuijthof, Ron M.A. Heeren, Pieter J. Emans, Berta Cillero-Pastor*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
37 Downloads (Pure)

Abstract

Mass spectrometry imaging (MSI) is a powerful technique enabling the visualization of the spatial distribution of different molecules in tissue biopsies with different pathologies. Sample handling and preparing adipose tissue for MSI is challenging and prone to molecular delocalization due to tissue melting. In this work, we developed a method for matrix-assisted laser desorption/ionization (MALDI)-MSI to study lipids in human infrapatellar fat pad (IPFP), a biomarker source in musculoskeletal pathologies, while preserving molecular spatial distribution. Cryosectioning at 15 μm with a temperature below −30 °C, thaw-mounting, and sublimation, was demonstrated to preserve IPFP's heterogeneous appearance and spatial distribution of lipids.

Original languageEnglish
Article number115018
JournalAnalytical biochemistry
Volume662
Early online date12 Dec 2022
DOIs
Publication statusPublished - 1 Feb 2023

Keywords

  • Hoffa's fat pad
  • Lipidomics
  • Delocalization
  • Adipose tissue

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