Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Amplification: An Overview

Joost Franciscus Swennenhuis, Leonardus Wendelinus Mathias Marie Terstappen

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Single cells are increasingly used to determine the heterogeneity of therapy targets in the genome during the course of a disease. The first challenge using single cells is to isolate these cells from the surrounding cells, especially when the targeted cells are rare. A number of techniques have been developed for this goal, each having specific limitations and possibilities. In this chapter, five of these techniques are discussed in the light of the isolation of circulating tumor cells (CTC) present at extremely low frequency in the blood of patients with metastatic cancer from the perspective of pre-enriched samples by means of CellSearch. The techniques described are micromanipulation, FACS, laser capture microdissection, DEPArray, and microfluidic solutions. All platforms are hampered with a low efficiency and differences in hands-on time and costs are the most important drivers for selection of the optimal platform.
Original languageEnglish
Title of host publicationWhole Genome Amplification: Methods and Protocols
EditorsThomas Kroneis
PublisherSpringer
Pages57-67
ISBN (Print)978-1-4939-2989-4
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer New York
Number1347

Keywords

  • IR-97571
  • METIS-312072

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