Scanning Probe Optical Tweezers: a new tool to study DNA-protein interactions

J.H.G. Huisstede

    Research output: ThesisPhD Thesis - Research UT, graduation UT

    57 Downloads (Pure)

    Abstract

    The main goal of the work described in this thesis is to construct a microscope in which OT and scanning probe microscopy (SPM) are combined, to be able to localize proteins while simultaneously controlling the tension within the DNA molecule. This apparatus enables the study of the effect of tension in the molecule on the functional properties of DNA-binding proteins. An advantage of the `Scanning Probe Optical Tweezers¿, i.e. SPOT-microscope, is that the DNA-protein interactions are not obstructed by the presence of a supporting surface as is the case for imaging using conventional SPM techniques.
    Original languageUndefined
    Awarding Institution
    • University of Twente
    Supervisors/Advisors
    • Subramaniam, Vinod, Supervisor
    • Bennink, Martin L., Advisor
    Award date20 Apr 2006
    Place of PublicationEnschede
    Publisher
    Print ISBNs90-365-2355-9
    Publication statusPublished - 20 Apr 2006

    Keywords

    • IR-55834
    • METIS-233865

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