Scanning Probe Optical Tweezers: a new tool to study DNA-protein interactions

J.H.G. Huisstede

Scanning Probe Optical Tweezers: a new tool to study DNA-protein interactions

J.H.G. Huisstede

Research output: Thesis › PhD Thesis - Research UT, graduation UT

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Abstract

The main goal of the work described in this thesis is to construct a microscope in which OT and scanning probe microscopy (SPM) are combined, to be able to localize proteins while simultaneously controlling the tension within the DNA molecule. This apparatus enables the study of the effect of tension in the molecule on the functional properties of DNA-binding proteins. An advantage of the `Scanning Probe Optical Tweezers¿, i.e. SPOT-microscope, is that the DNA-protein interactions are not obstructed by the presence of a supporting surface as is the case for imaging using conventional SPM techniques.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	University of Twente
Supervisors/Advisors	Subramaniam, Vinod, Supervisor Bennink, Martin L., Advisor
Award date	20 Apr 2006
Place of Publication	Enschede
Publisher	University of Twente
Print ISBNs	90-365-2355-9
Publication status	Published - 20 Apr 2006

Keywords

IR-55834
METIS-233865

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thesis_HuisstedeFinal published version, 5.38 MB

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title = "Scanning Probe Optical Tweezers: a new tool to study DNA-protein interactions",

abstract = "The main goal of the work described in this thesis is to construct a microscope in which OT and scanning probe microscopy (SPM) are combined, to be able to localize proteins while simultaneously controlling the tension within the DNA molecule. This apparatus enables the study of the effect of tension in the molecule on the functional properties of DNA-binding proteins. An advantage of the `Scanning Probe Optical Tweezers¿, i.e. SPOT-microscope, is that the DNA-protein interactions are not obstructed by the presence of a supporting surface as is the case for imaging using conventional SPM techniques.",

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year = "2006",

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N2 - The main goal of the work described in this thesis is to construct a microscope in which OT and scanning probe microscopy (SPM) are combined, to be able to localize proteins while simultaneously controlling the tension within the DNA molecule. This apparatus enables the study of the effect of tension in the molecule on the functional properties of DNA-binding proteins. An advantage of the `Scanning Probe Optical Tweezers¿, i.e. SPOT-microscope, is that the DNA-protein interactions are not obstructed by the presence of a supporting surface as is the case for imaging using conventional SPM techniques.

AB - The main goal of the work described in this thesis is to construct a microscope in which OT and scanning probe microscopy (SPM) are combined, to be able to localize proteins while simultaneously controlling the tension within the DNA molecule. This apparatus enables the study of the effect of tension in the molecule on the functional properties of DNA-binding proteins. An advantage of the `Scanning Probe Optical Tweezers¿, i.e. SPOT-microscope, is that the DNA-protein interactions are not obstructed by the presence of a supporting surface as is the case for imaging using conventional SPM techniques.

KW - IR-55834

KW - METIS-233865

M3 - PhD Thesis - Research UT, graduation UT

SN - 90-365-2355-9

PB - University of Twente

CY - Enschede

ER -

Scanning Probe Optical Tweezers: a new tool to study DNA-protein interactions

Abstract

Keywords

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