Scattered light fluorescence microscopy: Imaging through turbid layers

Ivo M. Vellekoop, Christof M. Aegerter

Research output: Contribution to journalArticleAcademicpeer-review

183 Citations (Scopus)

Abstract

A major limitation of any type of microscope is the penetration depth in turbid tissue. Here, we demonstrate a fundamentally novel kind of fluorescence microscope that images through optically thick turbid layers. The microscope uses scattered light, rather than light propagating along a straight path, for imaging with subwavelength resolution. Our method uses constructive interference to focus scattered laser light through the turbid layer. Microscopic fluorescent structures behind the layer were imaged by raster scanning the focus.

Original languageEnglish
Pages (from-to)1245-1247
Number of pages3
JournalOptics letters
Volume35
Issue number8
DOIs
Publication statusPublished - 15 Apr 2010
Externally publishedYes

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