SecB protein stabilizes a translocation-competent state of purified prePhoE protein

Ron Kusters, Truus de Vrije, Eefjan Breukink, Ben De Kruijff

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    Abstract

    Efficient translocation of pure precursor of PhoE protein (prePhoE) could be accomplished in an in vitro system consisting of only inverted Escherichia coli inner membrane vesicles, ATP, and SecA and SecB protein. In this in vitro system SecB and not trigger factor could stabilize a translocation-competent state of prePhoE. In contrast, translocation competency of proOmpA could be induced by both trigger factor and SecB protein, suggesting specificity in interactions between cytosolic factors and precursors in outer membrane protein translocation.
    Original languageEnglish
    Pages (from-to)20827-20830
    JournalJournal of biological chemistry
    Volume264
    Issue number35
    Publication statusPublished - 15 Dec 1989

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