Selective Immobilization of Protein Clusters on Polymeric Nanocraters

Andrea Valsesia; Pascal Colpo; Tarik Meziani; Frederic Bretagnol; Michael Lejeune; François Rossi; Anita Bouma; M.F. Garcia Parajo

doi:10.1002/adfm.200500653

Selective Immobilization of Protein Clusters on Polymeric Nanocraters

Andrea Valsesia, Pascal Colpo, Tarik Meziani, Frederic Bretagnol, Michael Lejeune, François Rossi, Anita Bouma, M.F. Garcia Parajo

Optical Sciences

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Abstract

A method for fabricating chemically nanopatterned surfaces based on a combination of colloidal lithography and plasma- enhanced chemical vapor deposition (PECVD) is presented. This method can be applied for the creation of different nanopatterns, and it is in principle not limited in patterning resolution. Nanocraters of poly(acrylic acid) (carboxylic moieties) surrounded by a matrix of poly(ethylene glycol) are fabricated. Chemical force microscopy demonstrates that the process is able to produce the expected surface chemical contrast. Finally, the carboxylic groups of the craters are activated in order to induce the covalent binding of fluorescent-labeled proteins. Fluorescence investigation using scanning confocal microscopy shows that the proteins are preferentially attached inside the functional craters.

Original language	Undefined
Pages (from-to)	1242-1246
Journal	Advanced functional materials
Volume	16
Issue number	9
DOIs	https://doi.org/10.1002/adfm.200500653
Publication status	Published - 2006

Keywords

Biosensors
Lithography
Nanopatterning
colloidal
Surface functionalization
IR-72164

Access to Document

10.1002/adfm.200500653

Cite this

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title = "Selective Immobilization of Protein Clusters on Polymeric Nanocraters",

abstract = "A method for fabricating chemically nanopatterned surfaces based on a combination of colloidal lithography and plasma- enhanced chemical vapor deposition (PECVD) is presented. This method can be applied for the creation of different nanopatterns, and it is in principle not limited in patterning resolution. Nanocraters of poly(acrylic acid) (carboxylic moieties) surrounded by a matrix of poly(ethylene glycol) are fabricated. Chemical force microscopy demonstrates that the process is able to produce the expected surface chemical contrast. Finally, the carboxylic groups of the craters are activated in order to induce the covalent binding of fluorescent-labeled proteins. Fluorescence investigation using scanning confocal microscopy shows that the proteins are preferentially attached inside the functional craters.",

keywords = "Biosensors, Lithography, Nanopatterning, colloidal, Surface functionalization, IR-72164",

author = "Andrea Valsesia and Pascal Colpo and Tarik Meziani and Frederic Bretagnol and Michael Lejeune and Fran{\c c}ois Rossi and Anita Bouma and {Garcia Parajo}, M.F.",

year = "2006",

doi = "10.1002/adfm.200500653",

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journal = "Advanced functional materials",

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TY - JOUR

T1 - Selective Immobilization of Protein Clusters on Polymeric Nanocraters

AU - Valsesia, Andrea

AU - Colpo, Pascal

AU - Meziani, Tarik

AU - Bretagnol, Frederic

AU - Lejeune, Michael

AU - Rossi, François

AU - Bouma, Anita

AU - Garcia Parajo, M.F.

PY - 2006

Y1 - 2006

N2 - A method for fabricating chemically nanopatterned surfaces based on a combination of colloidal lithography and plasma- enhanced chemical vapor deposition (PECVD) is presented. This method can be applied for the creation of different nanopatterns, and it is in principle not limited in patterning resolution. Nanocraters of poly(acrylic acid) (carboxylic moieties) surrounded by a matrix of poly(ethylene glycol) are fabricated. Chemical force microscopy demonstrates that the process is able to produce the expected surface chemical contrast. Finally, the carboxylic groups of the craters are activated in order to induce the covalent binding of fluorescent-labeled proteins. Fluorescence investigation using scanning confocal microscopy shows that the proteins are preferentially attached inside the functional craters.

AB - A method for fabricating chemically nanopatterned surfaces based on a combination of colloidal lithography and plasma- enhanced chemical vapor deposition (PECVD) is presented. This method can be applied for the creation of different nanopatterns, and it is in principle not limited in patterning resolution. Nanocraters of poly(acrylic acid) (carboxylic moieties) surrounded by a matrix of poly(ethylene glycol) are fabricated. Chemical force microscopy demonstrates that the process is able to produce the expected surface chemical contrast. Finally, the carboxylic groups of the craters are activated in order to induce the covalent binding of fluorescent-labeled proteins. Fluorescence investigation using scanning confocal microscopy shows that the proteins are preferentially attached inside the functional craters.

KW - Biosensors

KW - Lithography

KW - Nanopatterning

KW - colloidal

KW - Surface functionalization

KW - IR-72164

U2 - 10.1002/adfm.200500653

DO - 10.1002/adfm.200500653

M3 - Article

SN - 1616-301X

VL - 16

SP - 1242

EP - 1246

JO - Advanced functional materials

JF - Advanced functional materials

IS - 9

ER -