Self-organization of the bacterial cell-division protein FtsZ in confined environments

Sonia Mellouli; Begoña Monterroso; Esra Te Brinke; Venkatachalam Chokkalingam; Germán Rivas; Wilhelm T.S. Huck

doi:10.1039/c3sm51163d

Self-organization of the bacterial cell-division protein FtsZ in confined environments

Sonia Mellouli, Begoña Monterroso, Esra Te Brinke, Venkatachalam Chokkalingam, Germán Rivas^*, Wilhelm T.S. Huck

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

28 Citations (Scopus)

Abstract

We report a microfluidic approach to generate aqueous droplets in oil of different dimensionality, stabilized by a lipid monolayer, to systematically probe the polymerization of bacterial cell-division protein FtsZ into fibrous networks as a function of the concentrations of crowding agent, FtsZ, and GTP. FtsZ bundles confined in droplets were dynamic, and their distribution depended on the intrinsic properties of the system and restrictions imposed by the spatial boundaries.

Original language	English
Pages (from-to)	10493-10500
Number of pages	8
Journal	Soft matter
Volume	9
Issue number	44
DOIs	https://doi.org/10.1039/c3sm51163d
Publication status	Published - 28 Nov 2013
Externally published	Yes

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AU - Te Brinke, Esra

AU - Chokkalingam, Venkatachalam

AU - Rivas, Germán

AU - Huck, Wilhelm T.S.

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AB - We report a microfluidic approach to generate aqueous droplets in oil of different dimensionality, stabilized by a lipid monolayer, to systematically probe the polymerization of bacterial cell-division protein FtsZ into fibrous networks as a function of the concentrations of crowding agent, FtsZ, and GTP. FtsZ bundles confined in droplets were dynamic, and their distribution depended on the intrinsic properties of the system and restrictions imposed by the spatial boundaries.

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Self-organization of the bacterial cell-division protein FtsZ in confined environments

Abstract

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