Sensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA)

F. van Rossem, Johan G. Bomer, Hans L. de Boer, Yawar Abbas, Eddy de Weerd, Albert van den Berg, Séverine Le Gac

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)
2 Downloads (Pure)

Abstract

We report a novel sensing protocol based on ultra-short (< 5 ms) measurements, which is using a dedicated sensor consisting of an ultra-microelectrode array (UMEA) for monitoring the concentration in dissolved oxygen concentrations in solution. The UMEA sensor is fabricated from Pt and oxide-nitride-oxide (ONO) as an insulating material, and electrodes are recessed in a glass substrate. The UMEA sensor is operated in its linear regime, and the oxygen concentration in solution is derived in less than 5 ms from the slope of the measured current I as a function of 1/√t, t being the measurement time. To validate the proposed measurement protocol and to calibrate the sensor, variations in the concentration of dissolved oxygen are monitored simultaneously using the UMEA-based sensor and an external electrochemical sensor in a 10-mL wet-cell. An excellent agreement (R2 = 0.994) is found between the two sensors, and a sensitivity of 0.49 nAs−0.5/mg/L is determined for the UMEA sensor operated in this ultra-short measurement regime. Finally, and most importantly, the amount of oxygen consumed during the electrochemical measurements in this configuration is drastically reduced, i.e., by about 10 orders of magnitude, compared to a commercial electrochemical sensor, which is very valuable to monitor in situ the respiratory activity of microtissues in nL volumes as found in microfluidic systems.

Original languageEnglish
Pages (from-to)1008-1016
Number of pages9
JournalSensors and actuators. B: Chemical
Volume238
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint

Microelectrodes
Oxygen
Sensor arrays
sensors
oxygen
Electrochemical sensors
Sensors
Dissolved oxygen
Oxides
Insulating materials
Time measurement
Microfluidics
Nitrides
wet cells
oxides
Glass
Electrodes
Monitoring
insulation
Substrates

Keywords

  • Dissolved oxygen concentration
  • Minimal oxygen consumption
  • Real-time monitoring
  • Short measurement times
  • Ultra-microelectrode array (UMEA)

Cite this

@article{6b4f7325acb34d9da385bf146786e569,
title = "Sensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA)",
abstract = "We report a novel sensing protocol based on ultra-short (< 5 ms) measurements, which is using a dedicated sensor consisting of an ultra-microelectrode array (UMEA) for monitoring the concentration in dissolved oxygen concentrations in solution. The UMEA sensor is fabricated from Pt and oxide-nitride-oxide (ONO) as an insulating material, and electrodes are recessed in a glass substrate. The UMEA sensor is operated in its linear regime, and the oxygen concentration in solution is derived in less than 5 ms from the slope of the measured current I as a function of 1/√t, t being the measurement time. To validate the proposed measurement protocol and to calibrate the sensor, variations in the concentration of dissolved oxygen are monitored simultaneously using the UMEA-based sensor and an external electrochemical sensor in a 10-mL wet-cell. An excellent agreement (R2 = 0.994) is found between the two sensors, and a sensitivity of 0.49 nAs−0.5/mg/L is determined for the UMEA sensor operated in this ultra-short measurement regime. Finally, and most importantly, the amount of oxygen consumed during the electrochemical measurements in this configuration is drastically reduced, i.e., by about 10 orders of magnitude, compared to a commercial electrochemical sensor, which is very valuable to monitor in situ the respiratory activity of microtissues in nL volumes as found in microfluidic systems.",
keywords = "Dissolved oxygen concentration, Minimal oxygen consumption, Real-time monitoring, Short measurement times, Ultra-microelectrode array (UMEA)",
author = "{van Rossem}, F. and Bomer, {Johan G.} and {de Boer}, {Hans L.} and Yawar Abbas and {de Weerd}, Eddy and {van den Berg}, Albert and {Le Gac}, S{\'e}verine",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.snb.2016.06.156",
language = "English",
volume = "238",
pages = "1008--1016",
journal = "Sensors and actuators. B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

Sensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA). / van Rossem, F.; Bomer, Johan G.; de Boer, Hans L.; Abbas, Yawar; de Weerd, Eddy; van den Berg, Albert; Le Gac, Séverine.

In: Sensors and actuators. B: Chemical, Vol. 238, 01.01.2017, p. 1008-1016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Sensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA)

AU - van Rossem, F.

AU - Bomer, Johan G.

AU - de Boer, Hans L.

AU - Abbas, Yawar

AU - de Weerd, Eddy

AU - van den Berg, Albert

AU - Le Gac, Séverine

PY - 2017/1/1

Y1 - 2017/1/1

N2 - We report a novel sensing protocol based on ultra-short (< 5 ms) measurements, which is using a dedicated sensor consisting of an ultra-microelectrode array (UMEA) for monitoring the concentration in dissolved oxygen concentrations in solution. The UMEA sensor is fabricated from Pt and oxide-nitride-oxide (ONO) as an insulating material, and electrodes are recessed in a glass substrate. The UMEA sensor is operated in its linear regime, and the oxygen concentration in solution is derived in less than 5 ms from the slope of the measured current I as a function of 1/√t, t being the measurement time. To validate the proposed measurement protocol and to calibrate the sensor, variations in the concentration of dissolved oxygen are monitored simultaneously using the UMEA-based sensor and an external electrochemical sensor in a 10-mL wet-cell. An excellent agreement (R2 = 0.994) is found between the two sensors, and a sensitivity of 0.49 nAs−0.5/mg/L is determined for the UMEA sensor operated in this ultra-short measurement regime. Finally, and most importantly, the amount of oxygen consumed during the electrochemical measurements in this configuration is drastically reduced, i.e., by about 10 orders of magnitude, compared to a commercial electrochemical sensor, which is very valuable to monitor in situ the respiratory activity of microtissues in nL volumes as found in microfluidic systems.

AB - We report a novel sensing protocol based on ultra-short (< 5 ms) measurements, which is using a dedicated sensor consisting of an ultra-microelectrode array (UMEA) for monitoring the concentration in dissolved oxygen concentrations in solution. The UMEA sensor is fabricated from Pt and oxide-nitride-oxide (ONO) as an insulating material, and electrodes are recessed in a glass substrate. The UMEA sensor is operated in its linear regime, and the oxygen concentration in solution is derived in less than 5 ms from the slope of the measured current I as a function of 1/√t, t being the measurement time. To validate the proposed measurement protocol and to calibrate the sensor, variations in the concentration of dissolved oxygen are monitored simultaneously using the UMEA-based sensor and an external electrochemical sensor in a 10-mL wet-cell. An excellent agreement (R2 = 0.994) is found between the two sensors, and a sensitivity of 0.49 nAs−0.5/mg/L is determined for the UMEA sensor operated in this ultra-short measurement regime. Finally, and most importantly, the amount of oxygen consumed during the electrochemical measurements in this configuration is drastically reduced, i.e., by about 10 orders of magnitude, compared to a commercial electrochemical sensor, which is very valuable to monitor in situ the respiratory activity of microtissues in nL volumes as found in microfluidic systems.

KW - Dissolved oxygen concentration

KW - Minimal oxygen consumption

KW - Real-time monitoring

KW - Short measurement times

KW - Ultra-microelectrode array (UMEA)

UR - http://www.scopus.com/inward/record.url?scp=84989884728&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2016.06.156

DO - 10.1016/j.snb.2016.06.156

M3 - Article

VL - 238

SP - 1008

EP - 1016

JO - Sensors and actuators. B: Chemical

JF - Sensors and actuators. B: Chemical

SN - 0925-4005

ER -