Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration

Johanna Theodora Wilhelmina Berendsen; Jan C.T. Eijkel; Loes Irene Segerink

Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other articles. The new design circumvents this effect.

Original language	Undefined
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, MicroTAS 2016
Publisher	The Chemical and Biological Microsystems Society
Pages	1587-1588
Number of pages	2
ISBN (Print)	978-0-9798064-9-0
Publication status	Published - 16 Oct 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20 http://www.microtas2016.org/

Publication series

Name	International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS)
Publisher	The Chemical and Biological Microsystems Society
Number	--
ISSN (Print)	1556-5904

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016
Abbreviated title	MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16
Internet address	http://www.microtas2016.org/

Keywords

EWI-27516
IR-102626
METIS-319513

Access to Document

page1587

Cite this

Berendsen, J. T. W., Eijkel, J. C. T., & Segerink, L. I. (2016). Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration. In 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, MicroTAS 2016 (pp. 1587-1588). (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); No. --). The Chemical and Biological Microsystems Society.

Berendsen, Johanna Theodora Wilhelmina ; Eijkel, Jan C.T. ; Segerink, Loes Irene. / Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration. 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, MicroTAS 2016. The Chemical and Biological Microsystems Society, 2016. pp. 1587-1588 (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); --).

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abstract = "We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2\% and a separation efficiency of 100\%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other articles. The new design circumvents this effect.",

keywords = "EWI-27516, IR-102626, METIS-319513",

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Berendsen, JTW , Eijkel, JCT & Segerink, LI 2016, Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration. in 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, MicroTAS 2016. International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS), no. --, The Chemical and Biological Microsystems Society, pp. 1587-1588, 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016, Dublin, Ireland, 9/10/16.

Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration. / Berendsen, Johanna Theodora Wilhelmina ; Eijkel, Jan C.T.; Segerink, Loes Irene.
20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, MicroTAS 2016. The Chemical and Biological Microsystems Society, 2016. p. 1587-1588 (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); No. --).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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T1 - Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration

AU - Berendsen, Johanna Theodora Wilhelmina

AU - Eijkel, Jan C.T.

AU - Segerink, Loes Irene

N1 - Conference code: 20

PY - 2016/10/16

Y1 - 2016/10/16

N2 - We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other articles. The new design circumvents this effect.

AB - We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other articles. The new design circumvents this effect.

KW - EWI-27516

KW - IR-102626

KW - METIS-319513

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SN - 978-0-9798064-9-0

T3 - International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS)

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BT - 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, MicroTAS 2016

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Y2 - 9 October 2016 through 13 October 2016

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Berendsen JTW , Eijkel JCT , Segerink LI. Separation of spermatozoa with a combination of pinched flow fractionation and tangential filtration. In 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, MicroTAS 2016. The Chemical and Biological Microsystems Society. 2016. p. 1587-1588. (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); --).