TY - JOUR
T1 - Serial left-ventricular biopsy sampling using a minimally invasive trans-thoracic approach in adult dogs
AU - Donker, Dirk W.
AU - Maessen, Jos G.
AU - Spätjens, Roel L.H.M.G.
AU - Van Der Nagel, Theo
AU - De Jong, Monique
AU - Ramaekers, Frans C.
AU - Crijns, Harry J.G.M.
AU - Vos, Marc A.
AU - Volders, Paul G.A.
PY - 2007/9
Y1 - 2007/9
N2 - Myocardial biopsies are an increasingly important tool to unravel the molecular mechanisms of cardiac disease. We evaluate a novel minimally invasive trans-thoracic approach for left-ventricular (LV) intra-mural biopsies, which enables repetitive individual sampling in adult dogs. Forty three generally anaesthesised dogs were studied during sinus rhythm (SR, control) and multiple times after the induction of volume overload hypertrophy (complete atrioventricular block [AVB]). Through a small cutaneous incision, an automatic biopsy needle was advanced into the apicolateral LV, guided by fluoroscopy. Electrocardiography (ECG), LV pressure and echocardiography served to monitor the procedure. One hundred eighty-eight intra-mural LV biopsies were obtained in 82 serial experiments (usually SR, 1, 2 and 6 weeks AVB) with a maximum of 8 repeated biopsies. All biopsies (∼10 mm3) were suitable for simultaneous application of different cell-biological (light and electron microscopy, immunohistochemistry) and molecular techniques (PCR, Western blotting). In chronic experiments, repeated biopsy sampling did not influence haemodynamics, mechanics, electrocardiographic parameters or myocardial remodelling during SR or AVB. The rate of significant complications was as low as 4% of experiments. Minimally invasive sampling of LV needle biopsies enables serial assessment of myocardial remodelling using different molecular techniques in individual animals. The technique is safe and has no long-term effects on cardiac function or structure.
AB - Myocardial biopsies are an increasingly important tool to unravel the molecular mechanisms of cardiac disease. We evaluate a novel minimally invasive trans-thoracic approach for left-ventricular (LV) intra-mural biopsies, which enables repetitive individual sampling in adult dogs. Forty three generally anaesthesised dogs were studied during sinus rhythm (SR, control) and multiple times after the induction of volume overload hypertrophy (complete atrioventricular block [AVB]). Through a small cutaneous incision, an automatic biopsy needle was advanced into the apicolateral LV, guided by fluoroscopy. Electrocardiography (ECG), LV pressure and echocardiography served to monitor the procedure. One hundred eighty-eight intra-mural LV biopsies were obtained in 82 serial experiments (usually SR, 1, 2 and 6 weeks AVB) with a maximum of 8 repeated biopsies. All biopsies (∼10 mm3) were suitable for simultaneous application of different cell-biological (light and electron microscopy, immunohistochemistry) and molecular techniques (PCR, Western blotting). In chronic experiments, repeated biopsy sampling did not influence haemodynamics, mechanics, electrocardiographic parameters or myocardial remodelling during SR or AVB. The rate of significant complications was as low as 4% of experiments. Minimally invasive sampling of LV needle biopsies enables serial assessment of myocardial remodelling using different molecular techniques in individual animals. The technique is safe and has no long-term effects on cardiac function or structure.
KW - Instruments and techniques
KW - Myocardial biopsy
KW - Myocardial remodelling
KW - Ventricles
UR - http://www.scopus.com/inward/record.url?scp=34548297013&partnerID=8YFLogxK
U2 - 10.1007/s00424-007-0261-z
DO - 10.1007/s00424-007-0261-z
M3 - Article
C2 - 17505841
AN - SCOPUS:34548297013
VL - 454
SP - 1043
EP - 1051
JO - Pflügers Archiv : European journal of physiology
JF - Pflügers Archiv : European journal of physiology
SN - 0031-6768
IS - 6
ER -