Serial nonivasive photoacoustic imaging of neovascularization in tumor angiogenesis

R.I. Siphanto, K.K. Thumma, R.G.M. Kolkman, T.G. van Leeuwen, F.F.M. de Mul, J.W. van Neck, L.N.A. van Adrichem, W. Steenbergen

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Abstract

We present photoacoustic images of tumor neovascularization obtained over a 10-day period after subcutaneous inoculation of pancreatic tumor cells in a rat. The images were obtained from ultrasound generated by absorption in hemoglobin of short laser pulses at a wavelength of 1064 nm. The ultrasound signals were measured in reflection mode using a single scanning piezodetector, and images were reconstructed with a weighted delay-and-sum algorithm. Three-dimensional data visualize the development and quantify the extent of individual blood vessels around the growing tumor, blood concentration changes inside the tumor and growth in depth of the neovascularized region.
Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalOptics express
Volume13
Issue number1
DOIs
Publication statusPublished - 2005

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angiogenesis
tumors
blood vessels
hemoglobin
inoculation
rats
blood
scanning
pulses
wavelengths
lasers

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Siphanto, R. I., Thumma, K. K., Kolkman, R. G. M., van Leeuwen, T. G., de Mul, F. F. M., van Neck, J. W., ... Steenbergen, W. (2005). Serial nonivasive photoacoustic imaging of neovascularization in tumor angiogenesis. Optics express, 13(1), 89-95. https://doi.org/10.1364/OPEX.13.000089
Siphanto, R.I. ; Thumma, K.K. ; Kolkman, R.G.M. ; van Leeuwen, T.G. ; de Mul, F.F.M. ; van Neck, J.W. ; van Adrichem, L.N.A. ; Steenbergen, W. / Serial nonivasive photoacoustic imaging of neovascularization in tumor angiogenesis. In: Optics express. 2005 ; Vol. 13, No. 1. pp. 89-95.
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author = "R.I. Siphanto and K.K. Thumma and R.G.M. Kolkman and {van Leeuwen}, T.G. and {de Mul}, F.F.M. and {van Neck}, J.W. and {van Adrichem}, L.N.A. and W. Steenbergen",
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Siphanto, RI, Thumma, KK, Kolkman, RGM, van Leeuwen, TG, de Mul, FFM, van Neck, JW, van Adrichem, LNA & Steenbergen, W 2005, 'Serial nonivasive photoacoustic imaging of neovascularization in tumor angiogenesis', Optics express, vol. 13, no. 1, pp. 89-95. https://doi.org/10.1364/OPEX.13.000089

Serial nonivasive photoacoustic imaging of neovascularization in tumor angiogenesis. / Siphanto, R.I.; Thumma, K.K.; Kolkman, R.G.M.; van Leeuwen, T.G.; de Mul, F.F.M.; van Neck, J.W.; van Adrichem, L.N.A.; Steenbergen, W.

In: Optics express, Vol. 13, No. 1, 2005, p. 89-95.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Siphanto, R.I.

AU - Thumma, K.K.

AU - Kolkman, R.G.M.

AU - van Leeuwen, T.G.

AU - de Mul, F.F.M.

AU - van Neck, J.W.

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AU - Steenbergen, W.

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AB - We present photoacoustic images of tumor neovascularization obtained over a 10-day period after subcutaneous inoculation of pancreatic tumor cells in a rat. The images were obtained from ultrasound generated by absorption in hemoglobin of short laser pulses at a wavelength of 1064 nm. The ultrasound signals were measured in reflection mode using a single scanning piezodetector, and images were reconstructed with a weighted delay-and-sum algorithm. Three-dimensional data visualize the development and quantify the extent of individual blood vessels around the growing tumor, blood concentration changes inside the tumor and growth in depth of the neovascularized region.

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DO - 10.1364/OPEX.13.000089

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Siphanto RI, Thumma KK, Kolkman RGM, van Leeuwen TG, de Mul FFM, van Neck JW et al. Serial nonivasive photoacoustic imaging of neovascularization in tumor angiogenesis. Optics express. 2005;13(1):89-95. https://doi.org/10.1364/OPEX.13.000089