Setup for testing cameras for image guided surgery using a controlled NIR fluorescence mimicking light source and tissue phantom

Giota Georgiou, Rudolf M. Verdaasdonk*, Albert Van Der Veen, John H. Klaessens

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

7 Downloads (Pure)

Abstract

In the development of new near-infrared (NIR) fluorescence dyes for image guided surgery, there is a need for new NIR sensitive camera systems that can easily be adjusted to specific wavelength ranges in contrast the present clinical systems that are only optimized for ICG. To test alternative camera systems, a setup was developed to mimic the fluorescence light in a tissue phantom to measure the sensitivity and resolution. Selected narrow band NIR LED's were used to illuminate a 6mm diameter circular diffuse plate to create uniform intensity controllable light spot (μW-mW) as target/source for NIR camera's. Layers of (artificial) tissue with controlled thickness could be placed on the spot to mimic a fluorescent 'cancer' embedded in tissue. This setup was used to compare a range of NIR sensitive consumer's cameras for potential use in image guided surgery. The image of the spot obtained with the cameras was captured and analyzed using ImageJ software. Enhanced CCD night vision cameras were the most sensitive capable of showing intensities < 1 μW through 5 mm of tissue. However, there was no control over the automatic gain and hence noise level. NIR sensitive DSLR cameras proved relative less sensitive but could be fully manually controlled as to gain (ISO 25600) and exposure time and are therefore preferred for a clinical setting in combination with Wi-Fi remote control. The NIR fluorescence testing setup proved to be useful for camera testing and can be used for development and quality control of new NIR fluorescence guided surgery equipment.

Original languageEnglish
Title of host publicationMolecular-Guided Surgery
Subtitle of host publicationMolecules, Devices, and Applications III
EditorsSylvain Gioux, Brian W. Pogue
PublisherSPIE
ISBN (Electronic)9781510605398
DOIs
Publication statusPublished - 1 Jan 2017
Externally publishedYes

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10049
ISSN (Print)1605-7422

Keywords

  • Fluorescence
  • Image guided surgery
  • Phantom
  • Quality
  • Sensitivity

Fingerprint Dive into the research topics of 'Setup for testing cameras for image guided surgery using a controlled NIR fluorescence mimicking light source and tissue phantom'. Together they form a unique fingerprint.

  • Cite this

    Georgiou, G., Verdaasdonk, R. M., Van Der Veen, A., & Klaessens, J. H. (2017). Setup for testing cameras for image guided surgery using a controlled NIR fluorescence mimicking light source and tissue phantom. In S. Gioux, & B. W. Pogue (Eds.), Molecular-Guided Surgery: Molecules, Devices, and Applications III [100490E] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10049). SPIE. https://doi.org/10.1117/12.2253267