Shear stress induces a transient and VEGFR-2-dependent decrease in the motion of injected particles in endothelial cells

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Vascular endothelial cells form the inner lining of all blood vessels and play a central role in vessel physiology and disease. Endothelial cells are highly responsive to the mechanical stimulus of fluid shear stress that is exerted by blood flowing over their surface. In this study, the immediate micromechanical response of endothelial cells to physiological shear stress was characterized by tracking of ballistically injected, sub-micron, fluorescent particles. It was found that the mean squared displacement (MSD) of the particles decreases by a factor 1.5 within 10 min after the onset of shear stress. This decrease in particle motion is transient, since the MSD returns to control values within 15–30 min after the onset of shear. The immediate micromechanical stiffening is dependent on activation of the vascular endothelial growth factor receptor (VEGFR)-2, because inhibition of the receptor abrogates the micromechanical response. This work shows that the cytoskeleton is actively involved in the acute, functional response of endothelial cells to shear stress.
Original languageEnglish
Pages (from-to)179-192
Number of pages14
JournalBiorheology
Volume47
Issue number3-4
DOIs
Publication statusPublished - 2010

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Vascular Endothelial Growth Factor Receptor-2
Endothelial Cells
Cytoskeleton
Blood Vessels

Keywords

  • METIS-272745
  • EWI-19441

Cite this

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title = "Shear stress induces a transient and VEGFR-2-dependent decrease in the motion of injected particles in endothelial cells",
abstract = "Vascular endothelial cells form the inner lining of all blood vessels and play a central role in vessel physiology and disease. Endothelial cells are highly responsive to the mechanical stimulus of fluid shear stress that is exerted by blood flowing over their surface. In this study, the immediate micromechanical response of endothelial cells to physiological shear stress was characterized by tracking of ballistically injected, sub-micron, fluorescent particles. It was found that the mean squared displacement (MSD) of the particles decreases by a factor 1.5 within 10 min after the onset of shear stress. This decrease in particle motion is transient, since the MSD returns to control values within 15–30 min after the onset of shear. The immediate micromechanical stiffening is dependent on activation of the vascular endothelial growth factor receptor (VEGFR)-2, because inhibition of the receptor abrogates the micromechanical response. This work shows that the cytoskeleton is actively involved in the acute, functional response of endothelial cells to shear stress.",
keywords = "METIS-272745, EWI-19441",
author = "{van der Meer}, {Andries Dirk} and Y. Li and Duits, {Michael H.G.} and Poot, {Andreas A.} and Jan Feijen and I. Vermes",
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language = "English",
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pages = "179--192",
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Shear stress induces a transient and VEGFR-2-dependent decrease in the motion of injected particles in endothelial cells. / van der Meer, Andries Dirk; Li, Y.; Duits, Michael H.G.; Poot, Andreas A.; Feijen, Jan; Vermes, I.

In: Biorheology, Vol. 47, No. 3-4, 2010, p. 179-192.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Shear stress induces a transient and VEGFR-2-dependent decrease in the motion of injected particles in endothelial cells

AU - van der Meer, Andries Dirk

AU - Li, Y.

AU - Duits, Michael H.G.

AU - Poot, Andreas A.

AU - Feijen, Jan

AU - Vermes, I.

PY - 2010

Y1 - 2010

N2 - Vascular endothelial cells form the inner lining of all blood vessels and play a central role in vessel physiology and disease. Endothelial cells are highly responsive to the mechanical stimulus of fluid shear stress that is exerted by blood flowing over their surface. In this study, the immediate micromechanical response of endothelial cells to physiological shear stress was characterized by tracking of ballistically injected, sub-micron, fluorescent particles. It was found that the mean squared displacement (MSD) of the particles decreases by a factor 1.5 within 10 min after the onset of shear stress. This decrease in particle motion is transient, since the MSD returns to control values within 15–30 min after the onset of shear. The immediate micromechanical stiffening is dependent on activation of the vascular endothelial growth factor receptor (VEGFR)-2, because inhibition of the receptor abrogates the micromechanical response. This work shows that the cytoskeleton is actively involved in the acute, functional response of endothelial cells to shear stress.

AB - Vascular endothelial cells form the inner lining of all blood vessels and play a central role in vessel physiology and disease. Endothelial cells are highly responsive to the mechanical stimulus of fluid shear stress that is exerted by blood flowing over their surface. In this study, the immediate micromechanical response of endothelial cells to physiological shear stress was characterized by tracking of ballistically injected, sub-micron, fluorescent particles. It was found that the mean squared displacement (MSD) of the particles decreases by a factor 1.5 within 10 min after the onset of shear stress. This decrease in particle motion is transient, since the MSD returns to control values within 15–30 min after the onset of shear. The immediate micromechanical stiffening is dependent on activation of the vascular endothelial growth factor receptor (VEGFR)-2, because inhibition of the receptor abrogates the micromechanical response. This work shows that the cytoskeleton is actively involved in the acute, functional response of endothelial cells to shear stress.

KW - METIS-272745

KW - EWI-19441

U2 - 10.3233/BIR-2010-0569

DO - 10.3233/BIR-2010-0569

M3 - Article

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SP - 179

EP - 192

JO - Biorheology

JF - Biorheology

SN - 0006-355X

IS - 3-4

ER -