TY - JOUR
T1 - Silica-coated magnetic nanobeads in a flow enrichment target capture Halbach (FETCH) magnetic separation system for circulating tumor cell enrichment
AU - Liu, Peng
AU - He, Sitian
AU - Mentink, Anouk
AU - Hart, Pieter
AU - Wu, Yongjun
AU - Terstappen, Leon W.M.M.
AU - Jonkheijm, Pascal
AU - Stevens, Michiel
N1 - Publisher Copyright:
© 2025 The Author(s). FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
PY - 2025/3
Y1 - 2025/3
N2 - Detecting circulating tumor cells (CTCs) is challenging due to their low presence and heterogeneity. Traditional methods using EpCAM-based separation struggle with CTCs that have undergone epithelial-mesenchymal transition, as this results in lower EpCAM expression. This study presents the use of silica-coated magnetic nanobeads functionalized with streptavidin for CTC capture. Using the FETCH magnetic separation system, we validated the capture efficiency of our beads on tumor cells with varying EpCAM expression. Our beads showed superior capture rates for LNCaP (97%), PC3-9 (91%), PC3 (23%), A549 (22%), and T24 (8%) cells compared to commercial MojoSort™ beads. Despite slightly higher nonspecific binding than CellSearch, our beads demonstrated improved sensitivity for EpCAMlow cells, suggesting they have promise for enhanced CTC capture.
AB - Detecting circulating tumor cells (CTCs) is challenging due to their low presence and heterogeneity. Traditional methods using EpCAM-based separation struggle with CTCs that have undergone epithelial-mesenchymal transition, as this results in lower EpCAM expression. This study presents the use of silica-coated magnetic nanobeads functionalized with streptavidin for CTC capture. Using the FETCH magnetic separation system, we validated the capture efficiency of our beads on tumor cells with varying EpCAM expression. Our beads showed superior capture rates for LNCaP (97%), PC3-9 (91%), PC3 (23%), A549 (22%), and T24 (8%) cells compared to commercial MojoSort™ beads. Despite slightly higher nonspecific binding than CellSearch, our beads demonstrated improved sensitivity for EpCAMlow cells, suggesting they have promise for enhanced CTC capture.
KW - UT-Hybrid-D
KW - FETCH system
KW - magnetic nanobeads
KW - circulating tumor cells enrichment
UR - http://www.scopus.com/inward/record.url?scp=85214266361&partnerID=8YFLogxK
U2 - 10.1002/1873-3468.15094
DO - 10.1002/1873-3468.15094
M3 - Article
AN - SCOPUS:85214266361
SN - 0014-5793
VL - 599
SP - 724
EP - 738
JO - FEBS letters
JF - FEBS letters
IS - 5
ER -