Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode

L. Zhang; H. Gardeniers; C. Van Hoof; G. Desmet; W. De Malsche

Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode

L. Zhang
, H. Gardeniers
, C. Van Hoof
, G. Desmet
, W. De Malsche

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

This paper reports a novel method for separating double stranded (ds) DNA on silicon micro-pillar array chips, based on ion-pair reversed-phase (IP-RP) chromatography. Gradient elution mode has been used for on-chip DNA separation for the first time. With this mode, we have achieved separations of dsDNA fragments with larger lengths (up to 500 base pairs (bp)) and smaller length difference (down to 10bp) compared to the isocratic elution mode. Moreover, reproducibility and resolution loss of dsDNA separation have been investigated in the gradient mode.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	641-643
Number of pages	3
Publication status	Published - 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011 - Seattle, United States Duration: 2 Oct 2011 → 6 Oct 2011 Conference number: 15 https://microtasconferences.org/microtas2011/

Publication series

Name	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume	1

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011
Abbreviated title	MicroTAS
Country/Territory	United States
City	Seattle
Period	2/10/11 → 6/10/11
Internet address	https://microtasconferences.org/microtas2011/

Keywords

DNA chromatography
Gradient elution mode
Micropillar array chip

Cite this

Zhang, L., Gardeniers, H., Van Hoof, C., Desmet, G., & De Malsche, W. (2011). Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 641-643). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 1).

Zhang, L. ; Gardeniers, H. ; Van Hoof, C. et al. / Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 641-643 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).

@inproceedings{2dd9b33c6f124900bcebe4c35fc3b663,

title = "Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode",

abstract = "This paper reports a novel method for separating double stranded (ds) DNA on silicon micro-pillar array chips, based on ion-pair reversed-phase (IP-RP) chromatography. Gradient elution mode has been used for on-chip DNA separation for the first time. With this mode, we have achieved separations of dsDNA fragments with larger lengths (up to 500 base pairs (bp)) and smaller length difference (down to 10bp) compared to the isocratic elution mode. Moreover, reproducibility and resolution loss of dsDNA separation have been investigated in the gradient mode.",

keywords = "DNA chromatography, Gradient elution mode, Micropillar array chip",

author = "L. Zhang and H. Gardeniers and \{Van Hoof\}, C. and G. Desmet and \{De Malsche\}, W.",

year = "2011",

language = "English",

isbn = "9781618395955",

series = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

pages = "641--643",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011, MicroTAS ; Conference date: 02-10-2011 Through 06-10-2011",

url = "https://microtasconferences.org/microtas2011/",

}

Zhang, L, Gardeniers, H, Van Hoof, C, Desmet, G & De Malsche, W 2011, Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 1, pp. 641-643, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011, Seattle, Washington, United States, 2/10/11.

Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode. / Zhang, L.; Gardeniers, H.; Van Hoof, C. et al.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 641-643 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode

AU - Zhang, L.

AU - Gardeniers, H.

AU - Van Hoof, C.

AU - Desmet, G.

AU - De Malsche, W.

N1 - Conference code: 15

PY - 2011

Y1 - 2011

N2 - This paper reports a novel method for separating double stranded (ds) DNA on silicon micro-pillar array chips, based on ion-pair reversed-phase (IP-RP) chromatography. Gradient elution mode has been used for on-chip DNA separation for the first time. With this mode, we have achieved separations of dsDNA fragments with larger lengths (up to 500 base pairs (bp)) and smaller length difference (down to 10bp) compared to the isocratic elution mode. Moreover, reproducibility and resolution loss of dsDNA separation have been investigated in the gradient mode.

AB - This paper reports a novel method for separating double stranded (ds) DNA on silicon micro-pillar array chips, based on ion-pair reversed-phase (IP-RP) chromatography. Gradient elution mode has been used for on-chip DNA separation for the first time. With this mode, we have achieved separations of dsDNA fragments with larger lengths (up to 500 base pairs (bp)) and smaller length difference (down to 10bp) compared to the isocratic elution mode. Moreover, reproducibility and resolution loss of dsDNA separation have been investigated in the gradient mode.

KW - DNA chromatography

KW - Gradient elution mode

KW - Micropillar array chip

UR - https://www.scopus.com/pages/publications/84883799346

M3 - Conference contribution

AN - SCOPUS:84883799346

SN - 9781618395955

T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

SP - 641

EP - 643

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

Zhang L, Gardeniers H, Van Hoof C, Desmet G, De Malsche W. Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 641-643. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).

Silicon micropillar array chip for ion-pair reversed-phase DNA chromatography using gradient elution mode

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