Simple and stable transendothelial electrical resistance measurement in organs-on-chips

Marieke Willemijn van der Helm; Mathieu Odijk; Jean-Philippe Frimat; Jan C.T. Eijkel; Albert van den Berg; Loes Irene Segerink

Simple and stable transendothelial electrical resistance measurement in organs-on-chips

Marieke Willemijn van der Helm, Mathieu Odijk, Jean-Philippe Frimat, Jan C.T. Eijkel, Albert van den Berg, Loes Irene Segerink

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

2 Citations (Scopus)

2 Downloads (Pure)

Abstract

Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup

Original language	Undefined
Title of host publication	The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015)
Publisher	The Chemical and Biological Microsystems Society
Pages	771-773
Number of pages	3
ISBN (Print)	978-0-9798064-8-3
Publication status	Published - 25 Oct 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 Conference number: 19 http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Publication series

Name	Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Print)	1556-5904

Conference

Conference	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated title	MicroTas 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15
Internet address	http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Keywords

EWI-26552
IR-98702
METIS-315087

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Cite this

van der Helm, M. W., Odijk, M., Frimat, J-P., Eijkel, J. C. T., van den Berg, A., & Segerink, L. I. (2015). Simple and stable transendothelial electrical resistance measurement in organs-on-chips. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015) (pp. 771-773). (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS). The Chemical and Biological Microsystems Society. http://eprints.eemcs.utwente.nl/secure2/26552/01/0771_T.152b.pdf

van der Helm, Marieke Willemijn ; Odijk, Mathieu ; Frimat, Jean-Philippe et al. / Simple and stable transendothelial electrical resistance measurement in organs-on-chips. The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). The Chemical and Biological Microsystems Society, 2015. pp. 771-773 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).

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title = "Simple and stable transendothelial electrical resistance measurement in organs-on-chips",

abstract = "Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup",

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booktitle = "The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015)",

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van der Helm, MW, Odijk, M, Frimat, J-P, Eijkel, JCT , van den Berg, A & Segerink, LI 2015, Simple and stable transendothelial electrical resistance measurement in organs-on-chips. in The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS, The Chemical and Biological Microsystems Society, pp. 771-773, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15. <http://eprints.eemcs.utwente.nl/secure2/26552/01/0771_T.152b.pdf>

Simple and stable transendothelial electrical resistance measurement in organs-on-chips. / van der Helm, Marieke Willemijn; Odijk, Mathieu; Frimat, Jean-Philippe et al.

The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). The Chemical and Biological Microsystems Society, 2015. p. 771-773 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Simple and stable transendothelial electrical resistance measurement in organs-on-chips

AU - van der Helm, Marieke Willemijn

AU - Odijk, Mathieu

AU - Frimat, Jean-Philippe

AU - Eijkel, Jan C.T.

AU - van den Berg, Albert

AU - Segerink, Loes Irene

N1 - Conference code: 19

PY - 2015/10/25

Y1 - 2015/10/25

N2 - Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup

AB - Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup

KW - EWI-26552

KW - IR-98702

KW - METIS-315087

M3 - Conference contribution

SN - 978-0-9798064-8-3

T3 - Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS

SP - 771

EP - 773

BT - The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015)

PB - The Chemical and Biological Microsystems Society

Y2 - 25 October 2015 through 29 October 2015

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van der Helm MW, Odijk M, Frimat J-P, Eijkel JCT , van den Berg A , Segerink LI. Simple and stable transendothelial electrical resistance measurement in organs-on-chips. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). The Chemical and Biological Microsystems Society. 2015. p. 771-773. (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).