Simple and stable transendothelial electrical resistance measurement in organs-on-chips

Marieke Willemijn van der Helm, Mathieu Odijk, Jean-Philippe Frimat, Jan C.T. Eijkel, Albert van den Berg, Loes Irene Segerink

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    1 Citation (Scopus)
    2 Downloads (Pure)

    Abstract

    Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup
    Original languageUndefined
    Title of host publicationThe 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015)
    PublisherChemical and Biological Micro Systems Society
    Pages771-773
    Number of pages3
    ISBN (Print)978-0-9798064-8-3
    Publication statusPublished - 25 Oct 2015
    Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of
    Duration: 25 Oct 201529 Oct 2015
    Conference number: 19
    http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

    Publication series

    NameProceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS
    PublisherChemical and Biological Microsystems Society
    ISSN (Print)1556-5904

    Conference

    Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
    Abbreviated titleMicroTas 2015
    CountryKorea, Republic of
    CityGyeongju
    Period25/10/1529/10/15
    Internet address

    Keywords

    • EWI-26552
    • IR-98702
    • METIS-315087

    Cite this

    van der Helm, M. W., Odijk, M., Frimat, J-P., Eijkel, J. C. T., van den Berg, A., & Segerink, L. I. (2015). Simple and stable transendothelial electrical resistance measurement in organs-on-chips. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015) (pp. 771-773). (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS). Chemical and Biological Micro Systems Society.
    van der Helm, Marieke Willemijn ; Odijk, Mathieu ; Frimat, Jean-Philippe ; Eijkel, Jan C.T. ; van den Berg, Albert ; Segerink, Loes Irene. / Simple and stable transendothelial electrical resistance measurement in organs-on-chips. The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Chemical and Biological Micro Systems Society, 2015. pp. 771-773 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).
    @inproceedings{5ba70798f97b4a368efd9c1caf808d31,
    title = "Simple and stable transendothelial electrical resistance measurement in organs-on-chips",
    abstract = "Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup",
    keywords = "EWI-26552, IR-98702, METIS-315087",
    author = "{van der Helm}, {Marieke Willemijn} and Mathieu Odijk and Jean-Philippe Frimat and Eijkel, {Jan C.T.} and {van den Berg}, Albert and Segerink, {Loes Irene}",
    year = "2015",
    month = "10",
    day = "25",
    language = "Undefined",
    isbn = "978-0-9798064-8-3",
    series = "Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS",
    publisher = "Chemical and Biological Micro Systems Society",
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    }

    van der Helm, MW, Odijk, M, Frimat, J-P, Eijkel, JCT, van den Berg, A & Segerink, LI 2015, Simple and stable transendothelial electrical resistance measurement in organs-on-chips. in The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS, Chemical and Biological Micro Systems Society, pp. 771-773, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15.

    Simple and stable transendothelial electrical resistance measurement in organs-on-chips. / van der Helm, Marieke Willemijn; Odijk, Mathieu; Frimat, Jean-Philippe; Eijkel, Jan C.T.; van den Berg, Albert; Segerink, Loes Irene.

    The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Chemical and Biological Micro Systems Society, 2015. p. 771-773 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - Simple and stable transendothelial electrical resistance measurement in organs-on-chips

    AU - van der Helm, Marieke Willemijn

    AU - Odijk, Mathieu

    AU - Frimat, Jean-Philippe

    AU - Eijkel, Jan C.T.

    AU - van den Berg, Albert

    AU - Segerink, Loes Irene

    PY - 2015/10/25

    Y1 - 2015/10/25

    N2 - Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup

    AB - Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance. Therefore, we redesigned our blood-brain barrier on a chip model to be able to measure stable TEER with four electrodes positioned outside the culture area using a simple impedance measurement setup

    KW - EWI-26552

    KW - IR-98702

    KW - METIS-315087

    M3 - Conference contribution

    SN - 978-0-9798064-8-3

    T3 - Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS

    SP - 771

    EP - 773

    BT - The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015)

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    van der Helm MW, Odijk M, Frimat J-P, Eijkel JCT, van den Berg A, Segerink LI. Simple and stable transendothelial electrical resistance measurement in organs-on-chips. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Chemical and Biological Micro Systems Society. 2015. p. 771-773. (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).