Simulating clathrin mediated endocytosis

Wouter K. den Otter; M. Giani; Willem J. Briels

Simulating clathrin mediated endocytosis

Wouter K. den Otter, M. Giani, Willem J. Briels

Computational Chemical Physics

Research output: Contribution to conference › Poster

Abstract

Endocytosis is a process by which cells internalize large molecules. When adaptor proteins (AP2) bind to the cell membrane and cargo, they release a motif that binds clathrin proteins. The collected clathrin triskelia self-assemble into a lattice with pentagonal and hexagonal facets. The membrane starts to bend and eventually is wrapped around the cargo. A scission protein cuts the neck, the clathrin coat is removed, and the vesicle carries its cargo to an organelle for further processing. We are using highly coarse-grained patchy particles to simulate the formation of clathrin cages and clathrin coated membrane pits. The protein particles and membrane nodes are propagated by translational and rotational Brownian dynamics,5 subject to conservative forces and click-potentials turned on and off by Monte Carlo moves.6,7 We also explore the impact of APs on clathrin cage formation in solution by statistical mechanical considerations.

Original language	English
Publication status	Published - Apr 2017
Event	Molecular basis for membrane remodelling and organization - Roscoff, France Duration: 3 Apr 2017 → 7 Apr 2017 http://www.cnrs.fr/insb/cjm/2017/Bassereau_e.html

Conference

Conference	Molecular basis for membrane remodelling and organization
Country	France
City	Roscoff
Period	3/04/17 → 7/04/17
Internet address	http://www.cnrs.fr/insb/cjm/2017/Bassereau_e.html

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den Otter, W. K., Giani, M., & Briels, W. J. (2017). Simulating clathrin mediated endocytosis. Poster session presented at Molecular basis for membrane remodelling and organization, Roscoff, France.

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abstract = "Endocytosis is a process by which cells internalize large molecules. When adaptor proteins (AP2) bind to the cell membrane and cargo, they release a motif that binds clathrin proteins. The collected clathrin triskelia self-assemble into a lattice with pentagonal and hexagonal facets. The membrane starts to bend and eventually is wrapped around the cargo. A scission protein cuts the neck, the clathrin coat is removed, and the vesicle carries its cargo to an organelle for further processing. We are using highly coarse-grained patchy particles to simulate the formation of clathrin cages and clathrin coated membrane pits. The protein particles and membrane nodes are propagated by translational and rotational Brownian dynamics,5 subject to conservative forces and click-potentials turned on and off by Monte Carlo moves.6,7 We also explore the impact of APs on clathrin cage formation in solution by statistical mechanical considerations.",

author = "{den Otter}, {Wouter K.} and M. Giani and Briels, {Willem J.}",

year = "2017",

month = "4",

language = "English",

note = "Molecular basis for membrane remodelling and organization ; Conference date: 03-04-2017 Through 07-04-2017",

url = "http://www.cnrs.fr/insb/cjm/2017/Bassereau_e.html",

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T1 - Simulating clathrin mediated endocytosis

AU - den Otter, Wouter K.

AU - Giani, M.

AU - Briels, Willem J.

PY - 2017/4

Y1 - 2017/4

N2 - Endocytosis is a process by which cells internalize large molecules. When adaptor proteins (AP2) bind to the cell membrane and cargo, they release a motif that binds clathrin proteins. The collected clathrin triskelia self-assemble into a lattice with pentagonal and hexagonal facets. The membrane starts to bend and eventually is wrapped around the cargo. A scission protein cuts the neck, the clathrin coat is removed, and the vesicle carries its cargo to an organelle for further processing. We are using highly coarse-grained patchy particles to simulate the formation of clathrin cages and clathrin coated membrane pits. The protein particles and membrane nodes are propagated by translational and rotational Brownian dynamics,5 subject to conservative forces and click-potentials turned on and off by Monte Carlo moves.6,7 We also explore the impact of APs on clathrin cage formation in solution by statistical mechanical considerations.

AB - Endocytosis is a process by which cells internalize large molecules. When adaptor proteins (AP2) bind to the cell membrane and cargo, they release a motif that binds clathrin proteins. The collected clathrin triskelia self-assemble into a lattice with pentagonal and hexagonal facets. The membrane starts to bend and eventually is wrapped around the cargo. A scission protein cuts the neck, the clathrin coat is removed, and the vesicle carries its cargo to an organelle for further processing. We are using highly coarse-grained patchy particles to simulate the formation of clathrin cages and clathrin coated membrane pits. The protein particles and membrane nodes are propagated by translational and rotational Brownian dynamics,5 subject to conservative forces and click-potentials turned on and off by Monte Carlo moves.6,7 We also explore the impact of APs on clathrin cage formation in solution by statistical mechanical considerations.

M3 - Poster

ER -