Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development

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    Abstract

    Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.
    Original languageEnglish
    Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
    Place of PublicationSan Diego, CA
    PublisherChemical and Biochemical Society
    Pages2357-2359
    Number of pages3
    ISBN (Print)978-0-9798064-7-6
    Publication statusPublished - 26 Oct 2014
    Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - Henry B. Gonzalez Convention Center, San Antonio, United States
    Duration: 26 Oct 201430 Oct 2014
    Conference number: 18

    Publication series

    NameInternational Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS
    PublisherChemical and Biochemical Society
    Volume2014
    ISSN (Print)1556-5904

    Conference

    Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
    Abbreviated titleMicroTAS 2014
    CountryUnited States
    CitySan Antonio
    Period26/10/1430/10/14

    Keywords

    • Microfluidics
    • Bilayer lipid membrane
    • FRAP
    • Electrophysiology
    • Gramicidin

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