Abstract
Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Place of Publication | San Diego, CA |
| Publisher | Chemical and Biochemical Society |
| Pages | 2357-2359 |
| Number of pages | 3 |
| ISBN (Print) | 978-0-9798064-7-6 |
| Publication status | Published - 26 Oct 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 - Henry B. Gonzalez Convention Center, San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 Conference number: 18 |
Publication series
| Name | International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS |
|---|---|
| Publisher | Chemical and Biochemical Society |
| Volume | 2014 |
| ISSN (Print) | 1556-5904 |
Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 |
|---|---|
| Abbreviated title | MicroTAS 2014 |
| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Keywords
- Microfluidics
- Bilayer lipid membrane
- FRAP
- Electrophysiology
- Gramicidin