Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.
Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Place of PublicationSan Diego, CA
PublisherChemical and Biochemical Society
Pages2357-2359
Number of pages3
ISBN (Print)978-0-9798064-7-6
Publication statusPublished - 26 Oct 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014
Conference number: 18

Publication series

NameInternational Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS
PublisherChemical and Biochemical Society
Volume2014
ISSN (Print)1556-5904

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014
Abbreviated titleMicroTAS
CountryUnited States
CitySan Antonio
Period26/10/1430/10/14

Fingerprint

Gramicidin
Microfluidics
Drug Combinations
Ion Channels
Lab-On-A-Chip Devices
Cholesterol
Membrane Fluidity
Cell Membrane

Keywords

  • Microfluidics
  • Bilayer lipid membrane
  • FRAP
  • Electrophysiology
  • Gramicidin

Cite this

Stimberg, V. C., Prokofyev, A. V., de Boer, H. L., van den Berg, A., & le Gac, S. (2014). Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2357-2359). (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS; Vol. 2014). San Diego, CA: Chemical and Biochemical Society.
Stimberg, Verena C. ; Prokofyev, Alexander V. ; de Boer, Hans L. ; van den Berg, Albert ; le Gac, Severine. / Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego, CA : Chemical and Biochemical Society, 2014. pp. 2357-2359 (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS).
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abstract = "Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40{\%} mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.",
keywords = "Microfluidics, Bilayer lipid membrane, FRAP, Electrophysiology, Gramicidin",
author = "Stimberg, {Verena C.} and Prokofyev, {Alexander V.} and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",
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Stimberg, VC, Prokofyev, AV, de Boer, HL, van den Berg, A & le Gac, S 2014, Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS, vol. 2014, Chemical and Biochemical Society, San Diego, CA, pp. 2357-2359, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014, San Antonio, United States, 26/10/14.

Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. / Stimberg, Verena C.; Prokofyev, Alexander V.; de Boer, Hans L.; van den Berg, Albert; le Gac, Severine.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego, CA : Chemical and Biochemical Society, 2014. p. 2357-2359 (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS; Vol. 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development

AU - Stimberg, Verena C.

AU - Prokofyev, Alexander V.

AU - de Boer, Hans L.

AU - van den Berg, Albert

AU - le Gac, Severine

PY - 2014/10/26

Y1 - 2014/10/26

N2 - Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.

AB - Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.

KW - Microfluidics

KW - Bilayer lipid membrane

KW - FRAP

KW - Electrophysiology

KW - Gramicidin

M3 - Conference contribution

SN - 978-0-9798064-7-6

T3 - International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS

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EP - 2359

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biochemical Society

CY - San Diego, CA

ER -

Stimberg VC, Prokofyev AV, de Boer HL, van den Berg A, le Gac S. Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego, CA: Chemical and Biochemical Society. 2014. p. 2357-2359. (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS).