Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.
    Original languageEnglish
    Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
    Place of PublicationSan Diego, CA
    PublisherChemical and Biochemical Society
    Pages2357-2359
    Number of pages3
    ISBN (Print)978-0-9798064-7-6
    Publication statusPublished - 26 Oct 2014
    Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 - San Antonio, United States
    Duration: 26 Oct 201430 Oct 2014
    Conference number: 18

    Publication series

    NameInternational Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS
    PublisherChemical and Biochemical Society
    Volume2014
    ISSN (Print)1556-5904

    Conference

    Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014
    Abbreviated titleMicroTAS
    CountryUnited States
    CitySan Antonio
    Period26/10/1430/10/14

    Fingerprint

    Gramicidin
    Microfluidics
    Drug Combinations
    Ion Channels
    Lab-On-A-Chip Devices
    Cholesterol
    Membrane Fluidity
    Cell Membrane

    Keywords

    • Microfluidics
    • Bilayer lipid membrane
    • FRAP
    • Electrophysiology
    • Gramicidin

    Cite this

    Stimberg, V. C., Prokofyev, A. V., de Boer, H. L., van den Berg, A., & le Gac, S. (2014). Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2357-2359). (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS; Vol. 2014). San Diego, CA: Chemical and Biochemical Society.
    Stimberg, Verena C. ; Prokofyev, Alexander V. ; de Boer, Hans L. ; van den Berg, Albert ; le Gac, Severine. / Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego, CA : Chemical and Biochemical Society, 2014. pp. 2357-2359 (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS).
    @inproceedings{b1187a6867bf46ca90441c813430b533,
    title = "Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development",
    abstract = "Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40{\%} mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.",
    keywords = "Microfluidics, Bilayer lipid membrane, FRAP, Electrophysiology, Gramicidin",
    author = "Stimberg, {Verena C.} and Prokofyev, {Alexander V.} and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",
    year = "2014",
    month = "10",
    day = "26",
    language = "English",
    isbn = "978-0-9798064-7-6",
    series = "International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS",
    publisher = "Chemical and Biochemical Society",
    pages = "2357--2359",
    booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

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    Stimberg, VC, Prokofyev, AV, de Boer, HL, van den Berg, A & le Gac, S 2014, Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS, vol. 2014, Chemical and Biochemical Society, San Diego, CA, pp. 2357-2359, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014, San Antonio, United States, 26/10/14.

    Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. / Stimberg, Verena C.; Prokofyev, Alexander V.; de Boer, Hans L.; van den Berg, Albert; le Gac, Severine.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego, CA : Chemical and Biochemical Society, 2014. p. 2357-2359 (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS; Vol. 2014).

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development

    AU - Stimberg, Verena C.

    AU - Prokofyev, Alexander V.

    AU - de Boer, Hans L.

    AU - van den Berg, Albert

    AU - le Gac, Severine

    PY - 2014/10/26

    Y1 - 2014/10/26

    N2 - Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.

    AB - Alterations in the cell membrane properties can indirectly influence ion channel function. Here, we present a microfluidic device for simultaneous confocal and electrophysiological measurements to study the influence of cholesterol on bilayer models, while recording the activity of gramicidin. Our results reveal that the addition of cholesterol (15 & 40% mol) leads to thinning of the bilayer and a reduced fluidity. Concurrently, the gramicidin activity drops and the single channel lifetime becomes shorter, while both the measured decrease in bilayer thickness and fluidity are expected to cause the opposite, suggesting that other (mechanical) bilayer properties play an important role to modulate the gramicidin activity.

    KW - Microfluidics

    KW - Bilayer lipid membrane

    KW - FRAP

    KW - Electrophysiology

    KW - Gramicidin

    M3 - Conference contribution

    SN - 978-0-9798064-7-6

    T3 - International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS

    SP - 2357

    EP - 2359

    BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

    PB - Chemical and Biochemical Society

    CY - San Diego, CA

    ER -

    Stimberg VC, Prokofyev AV, de Boer HL, van den Berg A, le Gac S. Simultaneous confocal and electrophysiological assessment of membrane properties and ion channel activity in a microfluidic format - a powerful combination for drug development. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego, CA: Chemical and Biochemical Society. 2014. p. 2357-2359. (International Conference on Miniaturized Systems for Chemistry and Life Science, MicroTAS).