Abstract
We report a new and simple approach for preparative purification and fractionation of sub-10-kbp DNA molecules in a microfluidic device. Agarose gel with 1.2% concentration is used as the separation matrix. A 0.5-10 kbp DNA ladder is fractionated and separated from other ionic species in continuous flow within 2 minutes by periodically switching between two orthogonal electrical fields of strongly differing magnitude. The high-resolution separation is based on the variation in field-dependent mobility (FDM) for differently-sized DNA fragments.
Original language | Undefined |
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Title of host publication | 20th International conference on Miniaturized Systems for Chemistry and LifeSciences |
Publisher | The Chemical and Biological Microsystems Society |
Pages | 226-227 |
Number of pages | 2 |
ISBN (Print) | 978-0-9798064-9-0 |
Publication status | Published - 9 Oct 2016 |
Event | 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20 http://www.microtas2016.org/ |
Publication series
Name | International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS) |
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Publisher | the Chemical and Biological Microsystems Society |
Number | -- |
Volume | -- |
ISSN (Print) | 1556-5904 |
Conference
Conference | 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 |
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Abbreviated title | MicroTAS 2016 |
Country/Territory | Ireland |
City | Dublin |
Period | 9/10/16 → 13/10/16 |
Internet address |
Keywords
- METIS-317602
- IR-101001
- EWI-27334