Simultaneous DNA purification and fractionation in agarose gel on the micro-scale

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    Abstract

    We report a new and simple approach for preparative purification and fractionation of sub-10-kbp DNA molecules in a microfluidic device. Agarose gel with 1.2% concentration is used as the separation matrix. A 0.5-10 kbp DNA ladder is fractionated and separated from other ionic species in continuous flow within 2 minutes by periodically switching between two orthogonal electrical fields of strongly differing magnitude. The high-resolution separation is based on the variation in field-dependent mobility (FDM) for differently-sized DNA fragments.
    Original languageUndefined
    Title of host publication20th International conference on Miniaturized Systems for Chemistry and LifeSciences
    PublisherThe Chemical and Biological Microsystems Society
    Pages226-227
    Number of pages2
    ISBN (Print)978-0-9798064-9-0
    Publication statusPublished - 9 Oct 2016
    Event20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland
    Duration: 9 Oct 201613 Oct 2016
    Conference number: 20
    http://www.microtas2016.org/

    Publication series

    NameInternational conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS)
    Publisherthe Chemical and Biological Microsystems Society
    Number--
    Volume--
    ISSN (Print)1556-5904

    Conference

    Conference20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016
    Abbreviated titleMicroTAS 2016
    CountryIreland
    CityDublin
    Period9/10/1613/10/16
    Internet address

    Keywords

    • METIS-317602
    • IR-101001
    • EWI-27334

    Cite this

    Gümüscü, B., van den Berg, A., & Eijkel, J. C. T. (2016). Simultaneous DNA purification and fractionation in agarose gel on the micro-scale. In 20th International conference on Miniaturized Systems for Chemistry and LifeSciences (pp. 226-227). (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); Vol. --, No. --). The Chemical and Biological Microsystems Society.