Simultaneous DNA purification and fractionation in agarose gel on the micro-scale

B. Gümüscü; Albert van den Berg; Jan C.T. Eijkel

Simultaneous DNA purification and fractionation in agarose gel on the micro-scale

B. Gümüscü, Albert van den Berg, Jan C.T. Eijkel

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

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Abstract

We report a new and simple approach for preparative purification and fractionation of sub-10-kbp DNA molecules in a microfluidic device. Agarose gel with 1.2% concentration is used as the separation matrix. A 0.5-10 kbp DNA ladder is fractionated and separated from other ionic species in continuous flow within 2 minutes by periodically switching between two orthogonal electrical fields of strongly differing magnitude. The high-resolution separation is based on the variation in field-dependent mobility (FDM) for differently-sized DNA fragments.

Original language	Undefined
Title of host publication	20th International conference on Miniaturized Systems for Chemistry and LifeSciences
Publisher	The Chemical and Biological Microsystems Society
Pages	226-227
Number of pages	2
ISBN (Print)	978-0-9798064-9-0
Publication status	Published - 9 Oct 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20 http://www.microtas2016.org/

Publication series

Name	International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS)
Publisher	the Chemical and Biological Microsystems Society
Number	--
Volume	--
ISSN (Print)	1556-5904

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016
Abbreviated title	MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16
Internet address	http://www.microtas2016.org/

Keywords

METIS-317602
IR-101001
EWI-27334

Access to Document

http://www.microtas2016.org/

Cite this

Gümüscü, B., van den Berg, A., & Eijkel, J. C. T. (2016). Simultaneous DNA purification and fractionation in agarose gel on the micro-scale. In 20th International conference on Miniaturized Systems for Chemistry and LifeSciences (pp. 226-227). (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); Vol. --, No. --). The Chemical and Biological Microsystems Society. http://www.microtas2016.org/

Gümüscü, B. ; van den Berg, Albert ; Eijkel, Jan C.T. / Simultaneous DNA purification and fractionation in agarose gel on the micro-scale. 20th International conference on Miniaturized Systems for Chemistry and LifeSciences. The Chemical and Biological Microsystems Society, 2016. pp. 226-227 (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); --).

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abstract = "We report a new and simple approach for preparative purification and fractionation of sub-10-kbp DNA molecules in a microfluidic device. Agarose gel with 1.2% concentration is used as the separation matrix. A 0.5-10 kbp DNA ladder is fractionated and separated from other ionic species in continuous flow within 2 minutes by periodically switching between two orthogonal electrical fields of strongly differing magnitude. The high-resolution separation is based on the variation in field-dependent mobility (FDM) for differently-sized DNA fragments.",

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author = "B. G{\"u}m{\"u}sc{\"u} and {van den Berg}, Albert and Eijkel, {Jan C.T.}",

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series = "International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS)",

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Gümüscü, B, van den Berg, A & Eijkel, JCT 2016, Simultaneous DNA purification and fractionation in agarose gel on the micro-scale. in 20th International conference on Miniaturized Systems for Chemistry and LifeSciences. International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS), no. --, vol. --, The Chemical and Biological Microsystems Society, pp. 226-227, 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016, Dublin, Ireland, 9/10/16. <http://www.microtas2016.org/>

Simultaneous DNA purification and fractionation in agarose gel on the micro-scale. / Gümüscü, B.; van den Berg, Albert ; Eijkel, Jan C.T.

20th International conference on Miniaturized Systems for Chemistry and LifeSciences. The Chemical and Biological Microsystems Society, 2016. p. 226-227 (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); Vol. --, No. --).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

TY - GEN

T1 - Simultaneous DNA purification and fractionation in agarose gel on the micro-scale

AU - Gümüscü, B.

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - Conference code: 20

PY - 2016/10/9

Y1 - 2016/10/9

N2 - We report a new and simple approach for preparative purification and fractionation of sub-10-kbp DNA molecules in a microfluidic device. Agarose gel with 1.2% concentration is used as the separation matrix. A 0.5-10 kbp DNA ladder is fractionated and separated from other ionic species in continuous flow within 2 minutes by periodically switching between two orthogonal electrical fields of strongly differing magnitude. The high-resolution separation is based on the variation in field-dependent mobility (FDM) for differently-sized DNA fragments.

AB - We report a new and simple approach for preparative purification and fractionation of sub-10-kbp DNA molecules in a microfluidic device. Agarose gel with 1.2% concentration is used as the separation matrix. A 0.5-10 kbp DNA ladder is fractionated and separated from other ionic species in continuous flow within 2 minutes by periodically switching between two orthogonal electrical fields of strongly differing magnitude. The high-resolution separation is based on the variation in field-dependent mobility (FDM) for differently-sized DNA fragments.

KW - METIS-317602

KW - IR-101001

KW - EWI-27334

M3 - Conference contribution

SN - 978-0-9798064-9-0

T3 - International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS)

SP - 226

EP - 227

BT - 20th International conference on Miniaturized Systems for Chemistry and LifeSciences

PB - The Chemical and Biological Microsystems Society

Y2 - 9 October 2016 through 13 October 2016

ER -

Gümüscü B, van den Berg A , Eijkel JCT. Simultaneous DNA purification and fractionation in agarose gel on the micro-scale. In 20th International conference on Miniaturized Systems for Chemistry and LifeSciences. The Chemical and Biological Microsystems Society. 2016. p. 226-227. (International conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS); --).