Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

Maryou B. Lambros; George Seed; Semini Sumanasuriya; Veronica Gil; Mateus Crespo; Mariane Fontes; Rob Chandler; Niven Mehra; Gemma Fowler; Berni Ebbs; Penny Flohr; Susana Miranda; Wei Yuan; Alan Mackay; Ana Ferreira; Rita Pereira; Claudia Bertan; Ines Figueiredo; Ruth Riisnaes; Daniel Nava Rodrigues; Adam Sharp; Jane Goodall; Gunther Boysen; Suzanne Carreira; Diletta Bianchini; Pasquale Rescigno; Zafeiris Zafeiriou; Joanne Hunt; Deirdre Moloney; Lucy Hamilton; Rui P. Neves; Joost Swennenhuis; Kiki Andree; Nikolas H. Stoecklein; Leon W.M.M. Terstappen; Johann S. De Bono

doi:10.1158/1078-0432.CCR-18-0862

Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

Maryou B. Lambros, George Seed, Semini Sumanasuriya, Veronica Gil, Mateus Crespo, Mariane Fontes, Rob Chandler, Niven Mehra, Gemma Fowler, Berni Ebbs, Penny Flohr, Susana Miranda, Wei Yuan, Alan Mackay, Ana Ferreira, Rita Pereira, Claudia Bertan, Ines Figueiredo, Ruth Riisnaes, Daniel Nava RodriguesAdam Sharp, Jane Goodall, Gunther Boysen, Suzanne Carreira, Diletta Bianchini, Pasquale Rescigno, Zafeiris Zafeiriou, Joanne Hunt, Deirdre Moloney, Lucy Hamilton, Rui P. Neves, Joost Swennenhuis, Kiki Andree, Nikolas H. Stoecklein, Leon W.M.M. Terstappen, Johann S. De Bono (Corresponding Author)

Medical Cell Biophysics

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Abstract

Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.

Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.

Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.

Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution.

Original language	English
Pages (from-to)	5635-5644
Number of pages	10
Journal	Clinical cancer research
Volume	24
Issue number	22
Early online date	11 Sept 2018
DOIs	https://doi.org/10.1158/1078-0432.CCR-18-0862
Publication status	Published - 15 Nov 2018

Keywords

22/2 OA procedure

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1158/1078-0432.CCR-18-0862

ArticleAccepted author version, 2.85 MB
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Cite this

Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., ... De Bono, J. S. (2018). Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis. Clinical cancer research, 24(22), 5635-5644. https://doi.org/10.1158/1078-0432.CCR-18-0862

@article{ae159a0dfd6f448ba1d2237197526150,

title = "Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis",

abstract = "Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. ",

keywords = "22/2 OA procedure",

author = "Lambros, {Maryou B.} and George Seed and Semini Sumanasuriya and Veronica Gil and Mateus Crespo and Mariane Fontes and Rob Chandler and Niven Mehra and Gemma Fowler and Berni Ebbs and Penny Flohr and Susana Miranda and Wei Yuan and Alan Mackay and Ana Ferreira and Rita Pereira and Claudia Bertan and Ines Figueiredo and Ruth Riisnaes and Rodrigues, {Daniel Nava} and Adam Sharp and Jane Goodall and Gunther Boysen and Suzanne Carreira and Diletta Bianchini and Pasquale Rescigno and Zafeiris Zafeiriou and Joanne Hunt and Deirdre Moloney and Lucy Hamilton and Neves, {Rui P.} and Joost Swennenhuis and Kiki Andree and Stoecklein, {Nikolas H.} and Terstappen, {Leon W.M.M.} and {De Bono}, {Johann S.}",

year = "2018",

month = nov,

day = "15",

doi = "10.1158/1078-0432.CCR-18-0862",

language = "English",

volume = "24",

pages = "5635--5644",

journal = "Clinical cancer research",

issn = "1078-0432",

publisher = "American Association for Cancer Research Inc.",

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Lambros, MB, Seed, G, Sumanasuriya, S, Gil, V, Crespo, M, Fontes, M, Chandler, R, Mehra, N, Fowler, G, Ebbs, B, Flohr, P, Miranda, S, Yuan, W, Mackay, A, Ferreira, A, Pereira, R, Bertan, C, Figueiredo, I, Riisnaes, R, Rodrigues, DN, Sharp, A, Goodall, J, Boysen, G, Carreira, S, Bianchini, D, Rescigno, P, Zafeiriou, Z, Hunt, J, Moloney, D, Hamilton, L, Neves, RP, Swennenhuis, J, Andree, K, Stoecklein, NH, Terstappen, LWMM & De Bono, JS 2018, 'Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis', Clinical cancer research, vol. 24, no. 22, pp. 5635-5644. https://doi.org/10.1158/1078-0432.CCR-18-0862

TY - JOUR

T1 - Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

AU - Lambros, Maryou B.

AU - Seed, George

AU - Sumanasuriya, Semini

AU - Gil, Veronica

AU - Crespo, Mateus

AU - Fontes, Mariane

AU - Chandler, Rob

AU - Mehra, Niven

AU - Fowler, Gemma

AU - Ebbs, Berni

AU - Flohr, Penny

AU - Miranda, Susana

AU - Yuan, Wei

AU - Mackay, Alan

AU - Ferreira, Ana

AU - Pereira, Rita

AU - Bertan, Claudia

AU - Figueiredo, Ines

AU - Riisnaes, Ruth

AU - Rodrigues, Daniel Nava

AU - Sharp, Adam

AU - Goodall, Jane

AU - Boysen, Gunther

AU - Carreira, Suzanne

AU - Bianchini, Diletta

AU - Rescigno, Pasquale

AU - Zafeiriou, Zafeiris

AU - Hunt, Joanne

AU - Moloney, Deirdre

AU - Hamilton, Lucy

AU - Neves, Rui P.

AU - Swennenhuis, Joost

AU - Andree, Kiki

AU - Stoecklein, Nikolas H.

AU - Terstappen, Leon W.M.M.

AU - De Bono, Johann S.

PY - 2018/11/15

Y1 - 2018/11/15

N2 - Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution.

AB - Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution.

KW - 22/2 OA procedure

UR - http://www.scopus.com/inward/record.url?scp=85053552214&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/singlecell-analyses-prostate-cancer-liquid-biopsies-acquired-apheresis

U2 - 10.1158/1078-0432.CCR-18-0862

DO - 10.1158/1078-0432.CCR-18-0862

M3 - Article

SN - 1078-0432

VL - 24

SP - 5635

EP - 5644

JO - Clinical cancer research

JF - Clinical cancer research

IS - 22

ER -

Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

Abstract

Keywords

UN SDGs

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