Single-Cell Microgels: Technology, Challenges, and Applications

Tom Kamperman, Marcel Karperien, Séverine Le Gac, Jeroen Leijten* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)
49 Downloads (Pure)

Abstract

Single-cell-laden microgels effectively act as the engineered counterpart of the smallest living building block of life: a cell within its pericellular matrix. Recent breakthroughs have enabled the encapsulation of single cells in sub-100-μm microgels to provide physiologically relevant microniches with minimal mass transport limitations and favorable pharmacokinetic properties. Single-cell-laden microgels offer additional unprecedented advantages, including facile manipulation, culture, and analysis of individual cell within 3D microenvironments. Therefore, single-cell microgel technology is expected to be instrumental in many life science applications, including pharmacological screenings, regenerative medicine, and fundamental biological research. In this review, we discuss the latest trends, technical challenges, and breakthroughs, and present our vision of the future of single-cell microgel technology and its applications.

Original languageEnglish
Pages (from-to)850-865
Number of pages16
JournalTrends in biotechnology
Volume36
Issue number8
DOIs
Publication statusPublished - Aug 2018

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Technology
Pharmacokinetics
Encapsulation
Screening
Mass transfer
Regenerative Medicine
Biological Science Disciplines
Pharmacology
Research

Keywords

  • hydrogels
  • microenvironments
  • microfabrication
  • microfluidics
  • regenerative medicine
  • single cell analysis
  • 3D culture

Cite this

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title = "Single-Cell Microgels: Technology, Challenges, and Applications",
abstract = "Single-cell-laden microgels effectively act as the engineered counterpart of the smallest living building block of life: a cell within its pericellular matrix. Recent breakthroughs have enabled the encapsulation of single cells in sub-100-μm microgels to provide physiologically relevant microniches with minimal mass transport limitations and favorable pharmacokinetic properties. Single-cell-laden microgels offer additional unprecedented advantages, including facile manipulation, culture, and analysis of individual cell within 3D microenvironments. Therefore, single-cell microgel technology is expected to be instrumental in many life science applications, including pharmacological screenings, regenerative medicine, and fundamental biological research. In this review, we discuss the latest trends, technical challenges, and breakthroughs, and present our vision of the future of single-cell microgel technology and its applications.",
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Single-Cell Microgels : Technology, Challenges, and Applications. / Kamperman, Tom; Karperien, Marcel; Le Gac, Séverine; Leijten, Jeroen (Corresponding Author).

In: Trends in biotechnology, Vol. 36, No. 8, 08.2018, p. 850-865.

Research output: Contribution to journalArticleAcademicpeer-review

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T2 - Technology, Challenges, and Applications

AU - Kamperman, Tom

AU - Karperien, Marcel

AU - Le Gac, Séverine

AU - Leijten, Jeroen

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AB - Single-cell-laden microgels effectively act as the engineered counterpart of the smallest living building block of life: a cell within its pericellular matrix. Recent breakthroughs have enabled the encapsulation of single cells in sub-100-μm microgels to provide physiologically relevant microniches with minimal mass transport limitations and favorable pharmacokinetic properties. Single-cell-laden microgels offer additional unprecedented advantages, including facile manipulation, culture, and analysis of individual cell within 3D microenvironments. Therefore, single-cell microgel technology is expected to be instrumental in many life science applications, including pharmacological screenings, regenerative medicine, and fundamental biological research. In this review, we discuss the latest trends, technical challenges, and breakthroughs, and present our vision of the future of single-cell microgel technology and its applications.

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