Single-enzyme analysis in a droplet-based micro- and nanofluidic system

Rerngchai Arayanarakool, Lingling Shui, Servé W.M. Kengen, Albert van den Berg, Jan C.T. Eijkel

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    38 Citations (Scopus)

    Abstract

    The kinetic activity of individual enzyme molecules was determined in aqueous droplets generated in a nano- and microfluidic device. To avoid high background noise, the enzyme and substrate solution was confined into femtoliter carriers, achieving high product concentrations from single-molecule encapsulation. The tiny droplets (phi similar to 2.5-3 mu m) generated from this fluidic system were highly monodisperse, beneficial for an analysis of single enzyme activity. The method presented here allows to follow large numbers of individual droplets over time. The instrumental requirements are furthermore modest, since the small droplet size allows to use of standard microscope and standard Pyrex glass chips as well as the use of relatively high enzyme concentrations (nM range) for single molecule encapsulation
    Original languageUndefined
    Pages (from-to)1955-1962
    Number of pages8
    JournalLab on a chip
    Volume13
    Issue number10
    DOIs
    Publication statusPublished - 2013

    Keywords

    • EWI-23775
    • IR-87417
    • METIS-300045

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