Single-molecule enzymatic analysis in a droplet-based microfluidic system

Rerngchai Arayanarakool, Lingling Shui, S.W.M Kengen, Albert van den Berg, Jan C.T. Eijkel

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    1 Citation (Scopus)

    Abstract

    The kinetic activity of individual enzyme molecules was determined in aqueous droplets generated in a nano- and microfluidic device. To avoid high background noise, the enzyme and substrate solution was confined into femtolitre carriers to achieve single-molecule encapsulation. The tiny droplets (f~2.5-3 μm) generated from this fluidic system were highly monodisperse, beneficial for an analysis of single enzyme activity. Single-enzyme kinetics has previously been demonstrated in the microfluidic format in PDMS containers [1], surface-immobilized droplets [2], or liposomes [3]. Single- enzyme analysis in the droplet-based microfluidics was reported before [4] by encapsulating highly-diluted enzyme solution (110 fM) into large droplets (f~40 μm). But to our knowledge this is the first demonstration of the direct method of single enzyme encapsulation and analysis at high enzyme concentration in tiny droplets in a microfluidic system.
    Original languageUndefined
    Title of host publicationThe 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    EditorsT. Fujii, A. Hibara, S. Takeuchi, T. Fukuba
    PublisherChemical and Biological Micro Systems Society
    Pages1492-1494
    Number of pages3
    ISBN (Print)1556-5904
    Publication statusPublished - 29 Oct 2012
    Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan
    Duration: 28 Oct 20121 Nov 2012
    Conference number: 16

    Publication series

    NamemicroTAS
    PublisherChemical and Biological Microsystems Society
    ISSN (Print)1556-5904

    Conference

    Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
    Abbreviated titleMicroTAS
    CountryJapan
    CityOkinawa
    Period28/10/121/11/12

    Keywords

    • IR-83542
    • METIS-296177
    • EWI-22719

    Cite this