Single Molecule Fluorescence: from Physical Fascination to Biological Relevance

Research output: ThesisPhD Thesis - Research UT, graduation UT

8 Downloads (Pure)

Abstract

Confocal fluorescence microscopy is particularly well-known from the beautiful images that have been obtained with this technique from cells. Several cellular components could be nicely visualized simultaneously by staining them with different fluorophores. Not only for ensemble applications but also in single molecule research confocal fluorescence microscopy became a popular technique. In this thesis the possibilities are shown to study a complicated biological process, which is Nucleotide Excision Repair (NER), on the single molecule level with confocal fluorescence microscopy. In chapter one an introduction is given in single molecule fluorescence, properties of fluorescent labels and immobilization methods. Also the NER process is described and the configuration of the confocal fluorescence microscope used.
Original languageUndefined
Supervisors/Advisors
  • Greve, Jan, Supervisor
  • Otto, Cornelis , Advisor
  • Hoeijmkers, J.H.J., Supervisor
Award date16 May 2003
Place of PublicationEnschede
Publisher
Print ISBNs90-365-1901-2
Publication statusPublished - 16 May 2003

Keywords

  • METIS-212371
  • IR-41411

Cite this

@phdthesis{30966e9843084ad587a5b15e1c35ddd9,
title = "Single Molecule Fluorescence: from Physical Fascination to Biological Relevance",
abstract = "Confocal fluorescence microscopy is particularly well-known from the beautiful images that have been obtained with this technique from cells. Several cellular components could be nicely visualized simultaneously by staining them with different fluorophores. Not only for ensemble applications but also in single molecule research confocal fluorescence microscopy became a popular technique. In this thesis the possibilities are shown to study a complicated biological process, which is Nucleotide Excision Repair (NER), on the single molecule level with confocal fluorescence microscopy. In chapter one an introduction is given in single molecule fluorescence, properties of fluorescent labels and immobilization methods. Also the NER process is described and the configuration of the confocal fluorescence microscope used.",
keywords = "METIS-212371, IR-41411",
author = "Segers-Nolten, {Gezina M.J.}",
year = "2003",
month = "5",
day = "16",
language = "Undefined",
isbn = "90-365-1901-2",
publisher = "Universiteit Twente",

}

Single Molecule Fluorescence: from Physical Fascination to Biological Relevance. / Segers-Nolten, Gezina M.J.

Enschede : Universiteit Twente, 2003. 158 p.

Research output: ThesisPhD Thesis - Research UT, graduation UT

TY - THES

T1 - Single Molecule Fluorescence: from Physical Fascination to Biological Relevance

AU - Segers-Nolten, Gezina M.J.

PY - 2003/5/16

Y1 - 2003/5/16

N2 - Confocal fluorescence microscopy is particularly well-known from the beautiful images that have been obtained with this technique from cells. Several cellular components could be nicely visualized simultaneously by staining them with different fluorophores. Not only for ensemble applications but also in single molecule research confocal fluorescence microscopy became a popular technique. In this thesis the possibilities are shown to study a complicated biological process, which is Nucleotide Excision Repair (NER), on the single molecule level with confocal fluorescence microscopy. In chapter one an introduction is given in single molecule fluorescence, properties of fluorescent labels and immobilization methods. Also the NER process is described and the configuration of the confocal fluorescence microscope used.

AB - Confocal fluorescence microscopy is particularly well-known from the beautiful images that have been obtained with this technique from cells. Several cellular components could be nicely visualized simultaneously by staining them with different fluorophores. Not only for ensemble applications but also in single molecule research confocal fluorescence microscopy became a popular technique. In this thesis the possibilities are shown to study a complicated biological process, which is Nucleotide Excision Repair (NER), on the single molecule level with confocal fluorescence microscopy. In chapter one an introduction is given in single molecule fluorescence, properties of fluorescent labels and immobilization methods. Also the NER process is described and the configuration of the confocal fluorescence microscope used.

KW - METIS-212371

KW - IR-41411

M3 - PhD Thesis - Research UT, graduation UT

SN - 90-365-1901-2

PB - Universiteit Twente

CY - Enschede

ER -