Single-molecule fluorescence microscopy on nucleotide excision repair complexes using GFP fusion proteins

Gezina M.J. Segers-Nolten, Suzanne Rademakers, Wim Vermeulen, Aufrid T.M. Lenferink, Cornelis Otto, Jan Hoeijmakers, Jan Greve

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review


Scanning Confocal Fluorescence Microscopy is used for single molecule studies on DNA-protein complexes that occur in Nucleotide Excision Repair (NER). During DNA-damage elimination by the NER-pathway, complex protein structures assemble over DNA. It is our aim to resolve the architecture of these DNA-protein complexes and to study dynamic changes that occur in these structures. For this purpose NER- complexes are partly reconstituted onto DNA-substrates using NER-proteins fused to different Green Fluorescent Protein mutants. Here we describe the recombinant production of NER- GFP fusion proteins. Characterization of GFP fluorescence is shown together with results of GFP single molecule imaging. First results with NER-GFP fusion proteins are presented as well
Original languageUndefined
Title of host publicationLaser Microscopy
EditorsKarsten Koenig, Hans J. Tanke, Herbert Schneckenburger
Place of PublicationAmsterdam
PublisherSPIE Press
Number of pages9
ISBN (Print)0-8194-3820-0
Publication statusPublished - 11 Jan 2000
EventLaser microscopy - Amsterdam
Duration: 1 Dec 20001 Dec 2000

Publication series

NameSPIE proceedings
PublisherSPIE Press


ConferenceLaser microscopy
OtherDecember 2000


  • METIS-130078
  • IR-96419

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