Single-molecule fluorescence microscopy on nucleotide excision repair complexes using GFP fusion proteins

Gezina M.J. Segers-Nolten, Suzanne Rademakers, Wim Vermeulen, Aufrid T.M. Lenferink, Cornelis Otto, Jan Hoeijmakers, Jan Greve

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Scanning Confocal Fluorescence Microscopy is used for single molecule studies on DNA-protein complexes that occur in Nucleotide Excision Repair (NER). During DNA-damage elimination by the NER-pathway, complex protein structures assemble over DNA. It is our aim to resolve the architecture of these DNA-protein complexes and to study dynamic changes that occur in these structures. For this purpose NER- complexes are partly reconstituted onto DNA-substrates using NER-proteins fused to different Green Fluorescent Protein mutants. Here we describe the recombinant production of NER- GFP fusion proteins. Characterization of GFP fluorescence is shown together with results of GFP single molecule imaging. First results with NER-GFP fusion proteins are presented as well
Original languageUndefined
Title of host publicationLaser Microscopy
EditorsKarsten Koenig, Hans J. Tanke, Herbert Schneckenburger
Place of PublicationAmsterdam
PublisherSPIE Press
Pages97-105
Number of pages9
ISBN (Print)0-8194-3820-0
DOIs
Publication statusPublished - 11 Jan 2000
EventLaser microscopy - Amsterdam
Duration: 1 Dec 20001 Dec 2000

Publication series

NameSPIE proceedings
PublisherSPIE Press
Volume4164

Conference

ConferenceLaser microscopy
Period1/12/001/12/00
OtherDecember 2000

Keywords

  • METIS-130078
  • IR-96419

Cite this