Abstract
| Original language | Undefined |
|---|---|
| Pages (from-to) | 47-67 |
| Number of pages | 21 |
| Journal | Methods in enzymology |
| Volume | 521 |
| DOIs | |
| Publication status | Published - 2013 |
Keywords
- IR-89979
- METIS-297943
Cite this
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Single-molecule imaging technique to study the dynamic regulation of GPCR function at the plasma membrane. / Snaar-Jagalska, B.E.; Cambi, A.; Schmidt, T.; de Keijzer, S.
In: Methods in enzymology, Vol. 521, 2013, p. 47-67.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Single-molecule imaging technique to study the dynamic regulation of GPCR function at the plasma membrane
AU - Snaar-Jagalska, B.E.
AU - Cambi, A.
AU - Schmidt, T.
AU - de Keijzer, S.
PY - 2013
Y1 - 2013
N2 - The lateral diffusion of a G-protein-coupled receptor (GPCR) in the plasma membrane determines its interaction capabilities with downstream signaling molecules and critically modulates its function. Mechanisms that control GPCR mobility, like compartmentalization, enable a cell to fine-tune its response through local changes in the rate, duration, and extent of signaling. These processes are known to be highly dynamic and tightly regulated in time and space, usually not completely synchronized in time. Therefore, bulk studies such as protein biochemistry or conventional confocal microscopy will only yield information on the average properties of the interactions and are compromised by poor time resolution. Single-particle tracking (SPT) in living cells is a key approach to directly monitor the function of a GPCR within its natural environment and to obtain unprecedented detailed information about receptor mobility, binding kinetics, aggregation states, and domain formation. This review provides a detailed description on how to perform single GPCR tracking experiments.
AB - The lateral diffusion of a G-protein-coupled receptor (GPCR) in the plasma membrane determines its interaction capabilities with downstream signaling molecules and critically modulates its function. Mechanisms that control GPCR mobility, like compartmentalization, enable a cell to fine-tune its response through local changes in the rate, duration, and extent of signaling. These processes are known to be highly dynamic and tightly regulated in time and space, usually not completely synchronized in time. Therefore, bulk studies such as protein biochemistry or conventional confocal microscopy will only yield information on the average properties of the interactions and are compromised by poor time resolution. Single-particle tracking (SPT) in living cells is a key approach to directly monitor the function of a GPCR within its natural environment and to obtain unprecedented detailed information about receptor mobility, binding kinetics, aggregation states, and domain formation. This review provides a detailed description on how to perform single GPCR tracking experiments.
KW - IR-89979
KW - METIS-297943
U2 - 10.1016/B978-0-12-391862-8.00003-X
DO - 10.1016/B978-0-12-391862-8.00003-X
M3 - Article
VL - 521
SP - 47
EP - 67
JO - Methods in enzymology
JF - Methods in enzymology
SN - 0076-6879
ER -