Single Molecule Rotational and Translational Diffusion Observed by Near-field Scanning Optical Microscopy

A.G.T. Ruiter, J.A. Veerman, M.F. Garcia Parajo, N.F. van Hulst

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Abstract

We have observed rotational and translational diffusion of single molecules using a near-field scanning optical microscope with two polarization detection channels. The measurements were performed under ambient conditions with the molecules dispersed o­n glass or embedded in polymer. Tn successive images the fluorescence of single molecules was followed over about I h, with 10 ms integration time, until photodissociation, The position of single molecular fluorescence could be located with an accuracy of 1 nm. From the lateral diffusion of Rhodamine SG molecules o­n glass during successive images, a diffusion constant of (6.7 +/- 4.5) x 10(-15) cm(2)/s was determined, The orientation of the in-plane emission dipole of all molecules irt o­ne image could be directly determined with an accuracy of a few degrees by simultaneous detection in two perpendicular polarization directions. By rotating the excitation polarization we could selectively excite different sets of molecules and compare their in-plane absorption and emission dipole orientation. Monitoring Dil molecules in PMMA over 1 h, we found rotation of less than 10 degrees for the majority of molecules, while incidental fast rotation and transition to a dark state occurs. The fluorescence intensity was observed to be molecule dependent, which is an indication for out-of-plane orientation and different local photophysical environment.
Original languageUndefined
Pages (from-to)7318-7323
Number of pages6
JournalJournal of physical chemistry
Volume1997
Issue number101
DOIs
Publication statusPublished - 1997

Keywords

  • METIS-128611
  • IR-23812

Cite this

Ruiter, A.G.T. ; Veerman, J.A. ; Garcia Parajo, M.F. ; van Hulst, N.F. / Single Molecule Rotational and Translational Diffusion Observed by Near-field Scanning Optical Microscopy. In: Journal of physical chemistry. 1997 ; Vol. 1997, No. 101. pp. 7318-7323.
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title = "Single Molecule Rotational and Translational Diffusion Observed by Near-field Scanning Optical Microscopy",
abstract = "We have observed rotational and translational diffusion of single molecules using a near-field scanning optical microscope with two polarization detection channels. The measurements were performed under ambient conditions with the molecules dispersed o­n glass or embedded in polymer. Tn successive images the fluorescence of single molecules was followed over about I h, with 10 ms integration time, until photodissociation, The position of single molecular fluorescence could be located with an accuracy of 1 nm. From the lateral diffusion of Rhodamine SG molecules o­n glass during successive images, a diffusion constant of (6.7 +/- 4.5) x 10(-15) cm(2)/s was determined, The orientation of the in-plane emission dipole of all molecules irt o­ne image could be directly determined with an accuracy of a few degrees by simultaneous detection in two perpendicular polarization directions. By rotating the excitation polarization we could selectively excite different sets of molecules and compare their in-plane absorption and emission dipole orientation. Monitoring Dil molecules in PMMA over 1 h, we found rotation of less than 10 degrees for the majority of molecules, while incidental fast rotation and transition to a dark state occurs. The fluorescence intensity was observed to be molecule dependent, which is an indication for out-of-plane orientation and different local photophysical environment.",
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author = "A.G.T. Ruiter and J.A. Veerman and {Garcia Parajo}, M.F. and {van Hulst}, N.F.",
year = "1997",
doi = "10.1021/jp971066s",
language = "Undefined",
volume = "1997",
pages = "7318--7323",
journal = "Journal of physical chemistry",
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Single Molecule Rotational and Translational Diffusion Observed by Near-field Scanning Optical Microscopy. / Ruiter, A.G.T.; Veerman, J.A.; Garcia Parajo, M.F.; van Hulst, N.F.

In: Journal of physical chemistry, Vol. 1997, No. 101, 1997, p. 7318-7323.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Single Molecule Rotational and Translational Diffusion Observed by Near-field Scanning Optical Microscopy

AU - Ruiter, A.G.T.

AU - Veerman, J.A.

AU - Garcia Parajo, M.F.

AU - van Hulst, N.F.

PY - 1997

Y1 - 1997

N2 - We have observed rotational and translational diffusion of single molecules using a near-field scanning optical microscope with two polarization detection channels. The measurements were performed under ambient conditions with the molecules dispersed o­n glass or embedded in polymer. Tn successive images the fluorescence of single molecules was followed over about I h, with 10 ms integration time, until photodissociation, The position of single molecular fluorescence could be located with an accuracy of 1 nm. From the lateral diffusion of Rhodamine SG molecules o­n glass during successive images, a diffusion constant of (6.7 +/- 4.5) x 10(-15) cm(2)/s was determined, The orientation of the in-plane emission dipole of all molecules irt o­ne image could be directly determined with an accuracy of a few degrees by simultaneous detection in two perpendicular polarization directions. By rotating the excitation polarization we could selectively excite different sets of molecules and compare their in-plane absorption and emission dipole orientation. Monitoring Dil molecules in PMMA over 1 h, we found rotation of less than 10 degrees for the majority of molecules, while incidental fast rotation and transition to a dark state occurs. The fluorescence intensity was observed to be molecule dependent, which is an indication for out-of-plane orientation and different local photophysical environment.

AB - We have observed rotational and translational diffusion of single molecules using a near-field scanning optical microscope with two polarization detection channels. The measurements were performed under ambient conditions with the molecules dispersed o­n glass or embedded in polymer. Tn successive images the fluorescence of single molecules was followed over about I h, with 10 ms integration time, until photodissociation, The position of single molecular fluorescence could be located with an accuracy of 1 nm. From the lateral diffusion of Rhodamine SG molecules o­n glass during successive images, a diffusion constant of (6.7 +/- 4.5) x 10(-15) cm(2)/s was determined, The orientation of the in-plane emission dipole of all molecules irt o­ne image could be directly determined with an accuracy of a few degrees by simultaneous detection in two perpendicular polarization directions. By rotating the excitation polarization we could selectively excite different sets of molecules and compare their in-plane absorption and emission dipole orientation. Monitoring Dil molecules in PMMA over 1 h, we found rotation of less than 10 degrees for the majority of molecules, while incidental fast rotation and transition to a dark state occurs. The fluorescence intensity was observed to be molecule dependent, which is an indication for out-of-plane orientation and different local photophysical environment.

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U2 - 10.1021/jp971066s

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M3 - Article

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JO - Journal of physical chemistry

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