Single-molecule super-resolution imaging of microfabricated 3D substrates for 3D cell culture (Conference Presentation)

Clément Cabriel; Margoth Córdova-Castro; Erwin Berenschot; Amanda Dávila-Lezema; Kirsten Pondman; Séverine Le Gac; Niels Tas; Arturo Susarrey-Arce; Ignacio Izeddin

doi:10.1117/12.2648989

Single-molecule super-resolution imaging of microfabricated 3D substrates for 3D cell culture (Conference Presentation)

Clément Cabriel, Margoth Córdova-Castro, Erwin Berenschot, Amanda Dávila-Lezema, Kirsten Pondman, Séverine Le Gac, Niels Tas, Arturo Susarrey-Arce, Ignacio Izeddin

Research output: Contribution to conference › Other › Academic

Abstract

The potential of micro-and nanofabricated samples as a platform to modulate cell behavior using 3D physical cues has shown tremendous interest in cell differentiation. Investigating cell behavior and organization at the molecular level requires advanced imaging techniques. We produced multiscale 3D substrates with glass fractal pyramids composed of several generations of octahedra of decreasing sizes, which allow direct observation of cells in 3D SMLM. We show how these samples can be fluorescently labeled and used as a self-referenced sample for calibration and resolution measurements. Moreover, we perform quantitative 3D SMLM on cells growing on such fractal substrates, observing spheroid-like behavior.

Original language	English
DOIs	https://doi.org/10.1117/12.2648989
Publication status	Published - 15 Mar 2023
Event	SPIE BiOS 2023 - San Fransisco, United States Duration: 28 Jan 2023 → 3 Feb 2023

Conference

Conference	SPIE BiOS 2023
Abbreviated title	BiOS
Country/Territory	United States
City	San Fransisco
Period	28/01/23 → 3/02/23

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10.1117/12.2648989

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title = "Single-molecule super-resolution imaging of microfabricated 3D substrates for 3D cell culture (Conference Presentation)",

abstract = "The potential of micro-and nanofabricated samples as a platform to modulate cell behavior using 3D physical cues has shown tremendous interest in cell differentiation. Investigating cell behavior and organization at the molecular level requires advanced imaging techniques. We produced multiscale 3D substrates with glass fractal pyramids composed of several generations of octahedra of decreasing sizes, which allow direct observation of cells in 3D SMLM. We show how these samples can be fluorescently labeled and used as a self-referenced sample for calibration and resolution measurements. Moreover, we perform quantitative 3D SMLM on cells growing on such fractal substrates, observing spheroid-like behavior.",

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T1 - Single-molecule super-resolution imaging of microfabricated 3D substrates for 3D cell culture (Conference Presentation)

AU - Cabriel, Clément

AU - Córdova-Castro, Margoth

AU - Berenschot, Erwin

AU - Dávila-Lezema, Amanda

AU - Pondman, Kirsten

AU - Gac, Séverine Le

AU - Tas, Niels

AU - Susarrey-Arce, Arturo

AU - Izeddin, Ignacio

PY - 2023/3/15

Y1 - 2023/3/15

N2 - The potential of micro-and nanofabricated samples as a platform to modulate cell behavior using 3D physical cues has shown tremendous interest in cell differentiation. Investigating cell behavior and organization at the molecular level requires advanced imaging techniques. We produced multiscale 3D substrates with glass fractal pyramids composed of several generations of octahedra of decreasing sizes, which allow direct observation of cells in 3D SMLM. We show how these samples can be fluorescently labeled and used as a self-referenced sample for calibration and resolution measurements. Moreover, we perform quantitative 3D SMLM on cells growing on such fractal substrates, observing spheroid-like behavior.

AB - The potential of micro-and nanofabricated samples as a platform to modulate cell behavior using 3D physical cues has shown tremendous interest in cell differentiation. Investigating cell behavior and organization at the molecular level requires advanced imaging techniques. We produced multiscale 3D substrates with glass fractal pyramids composed of several generations of octahedra of decreasing sizes, which allow direct observation of cells in 3D SMLM. We show how these samples can be fluorescently labeled and used as a self-referenced sample for calibration and resolution measurements. Moreover, we perform quantitative 3D SMLM on cells growing on such fractal substrates, observing spheroid-like behavior.

U2 - 10.1117/12.2648989

DO - 10.1117/12.2648989

M3 - Other

T2 - SPIE BiOS 2023

Y2 - 28 January 2023 through 3 February 2023

ER -

Single-molecule super-resolution imaging of microfabricated 3D substrates for 3D cell culture (Conference Presentation)

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