Size matters: effects of PLGA-microsphere size in injectable CPC/PLGA on bone formation

Hongbing Liao, Rosa P. Félix Lanao, Jeroen J.J.P. van den Beucken, Nuo Zhou, Sanne K. Both, Joop G.C. Wolke, John A. Jansen

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The aim of this study was to evaluate the effect of PLGA microsphere dimensions on bone formation after injection of calcium phosphate cement (CPC)/PLGA in a guinea pig tibial intramedullarly model. To this end, injectable CPC/PLGA formulations were prepared using PLGA microspheres with either a small (~25 µm) or large (~100 µm) diameter, which were incorporated at a 20:80 ratio (wt%) within apatite CPC. Both CPC/PLGA formulations were injected into a marrow-ablated tibial intramedullary cavity and, after an implantation period of 12 weeks, histology and histomorphometry were used to address bone formation. The results demonstrated bone ingrowth throughout the entire scaffold material for both CPC/PLGA formulations upon PLGA microsphere degradation. More importantly, bone formation within the CPC matrix was > two-fold higher for CPC-PLGA with 25 µm PLGA microspheres. Additionally, the pattern of bone and marrow formation showed distinct differences related to PLGA microsphere dimension. In general, this study demonstrates that PLGA microsphere dimensions of ~25 µm, leading to pores of ~25 µm within CPC, are sufficient for bone ingrowth and allow substantial bone formation. Further, the results demonstrate that PLGA microsphere dimensions provide a tool to control bone formation for injectable CPC/PLGA bone substitutes.

Original languageEnglish
Pages (from-to)669-678
Number of pages10
JournalJournal of tissue engineering and regenerative medicine
Volume10
Issue number8
DOIs
Publication statusPublished - 1 Aug 2016
Externally publishedYes

Fingerprint

Bone cement
Calcium phosphate
Microspheres
Osteogenesis
Bone
Injections
calcium phosphate
polylactic acid-polyglycolic acid copolymer
Histology
Apatite
Bone Marrow
Scaffolds
Bone Substitutes
Apatites
Bone Cements
Degradation
Guinea Pigs

Keywords

  • animal model
  • biodegradation
  • calcium phosphate cement
  • microspheres
  • PLGA
  • size

Cite this

Liao, H., Félix Lanao, R. P., van den Beucken, J. J. J. P., Zhou, N., Both, S. K., Wolke, J. G. C., & Jansen, J. A. (2016). Size matters: effects of PLGA-microsphere size in injectable CPC/PLGA on bone formation. Journal of tissue engineering and regenerative medicine, 10(8), 669-678. https://doi.org/10.1002/term.1840
Liao, Hongbing ; Félix Lanao, Rosa P. ; van den Beucken, Jeroen J.J.P. ; Zhou, Nuo ; Both, Sanne K. ; Wolke, Joop G.C. ; Jansen, John A. / Size matters : effects of PLGA-microsphere size in injectable CPC/PLGA on bone formation. In: Journal of tissue engineering and regenerative medicine. 2016 ; Vol. 10, No. 8. pp. 669-678.
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abstract = "The aim of this study was to evaluate the effect of PLGA microsphere dimensions on bone formation after injection of calcium phosphate cement (CPC)/PLGA in a guinea pig tibial intramedullarly model. To this end, injectable CPC/PLGA formulations were prepared using PLGA microspheres with either a small (~25 µm) or large (~100 µm) diameter, which were incorporated at a 20:80 ratio (wt{\%}) within apatite CPC. Both CPC/PLGA formulations were injected into a marrow-ablated tibial intramedullary cavity and, after an implantation period of 12 weeks, histology and histomorphometry were used to address bone formation. The results demonstrated bone ingrowth throughout the entire scaffold material for both CPC/PLGA formulations upon PLGA microsphere degradation. More importantly, bone formation within the CPC matrix was > two-fold higher for CPC-PLGA with 25 µm PLGA microspheres. Additionally, the pattern of bone and marrow formation showed distinct differences related to PLGA microsphere dimension. In general, this study demonstrates that PLGA microsphere dimensions of ~25 µm, leading to pores of ~25 µm within CPC, are sufficient for bone ingrowth and allow substantial bone formation. Further, the results demonstrate that PLGA microsphere dimensions provide a tool to control bone formation for injectable CPC/PLGA bone substitutes.",
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Size matters : effects of PLGA-microsphere size in injectable CPC/PLGA on bone formation. / Liao, Hongbing; Félix Lanao, Rosa P.; van den Beucken, Jeroen J.J.P.; Zhou, Nuo; Both, Sanne K.; Wolke, Joop G.C.; Jansen, John A.

In: Journal of tissue engineering and regenerative medicine, Vol. 10, No. 8, 01.08.2016, p. 669-678.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Size matters

T2 - effects of PLGA-microsphere size in injectable CPC/PLGA on bone formation

AU - Liao, Hongbing

AU - Félix Lanao, Rosa P.

AU - van den Beucken, Jeroen J.J.P.

AU - Zhou, Nuo

AU - Both, Sanne K.

AU - Wolke, Joop G.C.

AU - Jansen, John A.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - The aim of this study was to evaluate the effect of PLGA microsphere dimensions on bone formation after injection of calcium phosphate cement (CPC)/PLGA in a guinea pig tibial intramedullarly model. To this end, injectable CPC/PLGA formulations were prepared using PLGA microspheres with either a small (~25 µm) or large (~100 µm) diameter, which were incorporated at a 20:80 ratio (wt%) within apatite CPC. Both CPC/PLGA formulations were injected into a marrow-ablated tibial intramedullary cavity and, after an implantation period of 12 weeks, histology and histomorphometry were used to address bone formation. The results demonstrated bone ingrowth throughout the entire scaffold material for both CPC/PLGA formulations upon PLGA microsphere degradation. More importantly, bone formation within the CPC matrix was > two-fold higher for CPC-PLGA with 25 µm PLGA microspheres. Additionally, the pattern of bone and marrow formation showed distinct differences related to PLGA microsphere dimension. In general, this study demonstrates that PLGA microsphere dimensions of ~25 µm, leading to pores of ~25 µm within CPC, are sufficient for bone ingrowth and allow substantial bone formation. Further, the results demonstrate that PLGA microsphere dimensions provide a tool to control bone formation for injectable CPC/PLGA bone substitutes.

AB - The aim of this study was to evaluate the effect of PLGA microsphere dimensions on bone formation after injection of calcium phosphate cement (CPC)/PLGA in a guinea pig tibial intramedullarly model. To this end, injectable CPC/PLGA formulations were prepared using PLGA microspheres with either a small (~25 µm) or large (~100 µm) diameter, which were incorporated at a 20:80 ratio (wt%) within apatite CPC. Both CPC/PLGA formulations were injected into a marrow-ablated tibial intramedullary cavity and, after an implantation period of 12 weeks, histology and histomorphometry were used to address bone formation. The results demonstrated bone ingrowth throughout the entire scaffold material for both CPC/PLGA formulations upon PLGA microsphere degradation. More importantly, bone formation within the CPC matrix was > two-fold higher for CPC-PLGA with 25 µm PLGA microspheres. Additionally, the pattern of bone and marrow formation showed distinct differences related to PLGA microsphere dimension. In general, this study demonstrates that PLGA microsphere dimensions of ~25 µm, leading to pores of ~25 µm within CPC, are sufficient for bone ingrowth and allow substantial bone formation. Further, the results demonstrate that PLGA microsphere dimensions provide a tool to control bone formation for injectable CPC/PLGA bone substitutes.

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KW - biodegradation

KW - calcium phosphate cement

KW - microspheres

KW - PLGA

KW - size

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DO - 10.1002/term.1840

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JO - Journal of tissue engineering and regenerative medicine

JF - Journal of tissue engineering and regenerative medicine

SN - 1932-6254

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