Abstract
The aim of this study was to evaluate the effect of PLGA microsphere dimensions on bone formation after injection of calcium phosphate cement (CPC)/PLGA in a guinea pig tibial intramedullarly model. To this end, injectable CPC/PLGA formulations were prepared using PLGA microspheres with either a small (~25 µm) or large (~100 µm) diameter, which were incorporated at a 20:80 ratio (wt%) within apatite CPC. Both CPC/PLGA formulations were injected into a marrow-ablated tibial intramedullary cavity and, after an implantation period of 12 weeks, histology and histomorphometry were used to address bone formation. The results demonstrated bone ingrowth throughout the entire scaffold material for both CPC/PLGA formulations upon PLGA microsphere degradation. More importantly, bone formation within the CPC matrix was > two-fold higher for CPC-PLGA with 25 µm PLGA microspheres. Additionally, the pattern of bone and marrow formation showed distinct differences related to PLGA microsphere dimension. In general, this study demonstrates that PLGA microsphere dimensions of ~25 µm, leading to pores of ~25 µm within CPC, are sufficient for bone ingrowth and allow substantial bone formation. Further, the results demonstrate that PLGA microsphere dimensions provide a tool to control bone formation for injectable CPC/PLGA bone substitutes.
| Original language | English |
|---|---|
| Pages (from-to) | 669-678 |
| Number of pages | 10 |
| Journal | Journal of tissue engineering and regenerative medicine |
| Volume | 10 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 1 Aug 2016 |
| Externally published | Yes |
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Keywords
- animal model
- biodegradation
- calcium phosphate cement
- microspheres
- PLGA
- size
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Size matters : effects of PLGA-microsphere size in injectable CPC/PLGA on bone formation. / Liao, Hongbing; Félix Lanao, Rosa P.; van den Beucken, Jeroen J.J.P.; Zhou, Nuo; Both, Sanne K.; Wolke, Joop G.C.; Jansen, John A.
In: Journal of tissue engineering and regenerative medicine, Vol. 10, No. 8, 01.08.2016, p. 669-678.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Size matters
T2 - effects of PLGA-microsphere size in injectable CPC/PLGA on bone formation
AU - Liao, Hongbing
AU - Félix Lanao, Rosa P.
AU - van den Beucken, Jeroen J.J.P.
AU - Zhou, Nuo
AU - Both, Sanne K.
AU - Wolke, Joop G.C.
AU - Jansen, John A.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - The aim of this study was to evaluate the effect of PLGA microsphere dimensions on bone formation after injection of calcium phosphate cement (CPC)/PLGA in a guinea pig tibial intramedullarly model. To this end, injectable CPC/PLGA formulations were prepared using PLGA microspheres with either a small (~25 µm) or large (~100 µm) diameter, which were incorporated at a 20:80 ratio (wt%) within apatite CPC. Both CPC/PLGA formulations were injected into a marrow-ablated tibial intramedullary cavity and, after an implantation period of 12 weeks, histology and histomorphometry were used to address bone formation. The results demonstrated bone ingrowth throughout the entire scaffold material for both CPC/PLGA formulations upon PLGA microsphere degradation. More importantly, bone formation within the CPC matrix was > two-fold higher for CPC-PLGA with 25 µm PLGA microspheres. Additionally, the pattern of bone and marrow formation showed distinct differences related to PLGA microsphere dimension. In general, this study demonstrates that PLGA microsphere dimensions of ~25 µm, leading to pores of ~25 µm within CPC, are sufficient for bone ingrowth and allow substantial bone formation. Further, the results demonstrate that PLGA microsphere dimensions provide a tool to control bone formation for injectable CPC/PLGA bone substitutes.
AB - The aim of this study was to evaluate the effect of PLGA microsphere dimensions on bone formation after injection of calcium phosphate cement (CPC)/PLGA in a guinea pig tibial intramedullarly model. To this end, injectable CPC/PLGA formulations were prepared using PLGA microspheres with either a small (~25 µm) or large (~100 µm) diameter, which were incorporated at a 20:80 ratio (wt%) within apatite CPC. Both CPC/PLGA formulations were injected into a marrow-ablated tibial intramedullary cavity and, after an implantation period of 12 weeks, histology and histomorphometry were used to address bone formation. The results demonstrated bone ingrowth throughout the entire scaffold material for both CPC/PLGA formulations upon PLGA microsphere degradation. More importantly, bone formation within the CPC matrix was > two-fold higher for CPC-PLGA with 25 µm PLGA microspheres. Additionally, the pattern of bone and marrow formation showed distinct differences related to PLGA microsphere dimension. In general, this study demonstrates that PLGA microsphere dimensions of ~25 µm, leading to pores of ~25 µm within CPC, are sufficient for bone ingrowth and allow substantial bone formation. Further, the results demonstrate that PLGA microsphere dimensions provide a tool to control bone formation for injectable CPC/PLGA bone substitutes.
KW - animal model
KW - biodegradation
KW - calcium phosphate cement
KW - microspheres
KW - PLGA
KW - size
UR - http://www.scopus.com/inward/record.url?scp=85027942616&partnerID=8YFLogxK
U2 - 10.1002/term.1840
DO - 10.1002/term.1840
M3 - Article
VL - 10
SP - 669
EP - 678
JO - Journal of tissue engineering and regenerative medicine
JF - Journal of tissue engineering and regenerative medicine
SN - 1932-6254
IS - 8
ER -