Small molecule absorption by PDMS in the context of drug response bioassays

B.J. van Meer, H. de Vries, K.S.A. Firth, Jasper van Weerd, L.G.J. Tertoolen, H.B.J. Karperien, Pascal Jonkheijm, C. Denning, A.P. IJzerman, C.L. Mummery

Research output: Contribution to journalArticleAcademicpeer-review

43 Citations (Scopus)
44 Downloads (Pure)

Abstract

The polymer polydimethylsiloxane (PDMS) is widely used to build microfluidic devices compatible with cell culture. Whilst convenient in manufacture, PDMS has the disadvantage that it can absorb small molecules such as drugs. In microfluidic devices like "Organs-on-Chip", designed to examine cell behavior and test the effects of drugs, this might impact drug bioavailability. Here we developed an assay to compare the absorption of a test set of four cardiac drugs by PDMS based on measuring the residual non-absorbed compound by High Pressure Liquid Chromatography (HPLC). We showed that absorption was variable and time dependent and not determined exclusively by hydrophobicity as claimed previously. We demonstrated that two commercially available lipophilic coatings and the presence of cells affected absorption. The use of lipophilic coatings may be useful in preventing small molecule absorption by PDMS.
Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalBiochemical and biophysical research communications
Volume482
Issue number2
DOIs
Publication statusPublished - 2017

Fingerprint

Bioassay
Lab-On-A-Chip Devices
Biological Assay
Molecules
Microfluidics
Pharmaceutical Preparations
High pressure liquid chromatography
Coatings
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Cell culture
Biological Availability
Assays
Polymers
Cell Culture Techniques
High Pressure Liquid Chromatography
baysilon

Keywords

  • METIS-321052
  • IR-103086

Cite this

van Meer, B.J. ; de Vries, H. ; Firth, K.S.A. ; van Weerd, Jasper ; Tertoolen, L.G.J. ; Karperien, H.B.J. ; Jonkheijm, Pascal ; Denning, C. ; IJzerman, A.P. ; Mummery, C.L. / Small molecule absorption by PDMS in the context of drug response bioassays. In: Biochemical and biophysical research communications. 2017 ; Vol. 482, No. 2. pp. 323-328.
@article{1d2d7399a48c450b88dc67f27f45d404,
title = "Small molecule absorption by PDMS in the context of drug response bioassays",
abstract = "The polymer polydimethylsiloxane (PDMS) is widely used to build microfluidic devices compatible with cell culture. Whilst convenient in manufacture, PDMS has the disadvantage that it can absorb small molecules such as drugs. In microfluidic devices like {"}Organs-on-Chip{"}, designed to examine cell behavior and test the effects of drugs, this might impact drug bioavailability. Here we developed an assay to compare the absorption of a test set of four cardiac drugs by PDMS based on measuring the residual non-absorbed compound by High Pressure Liquid Chromatography (HPLC). We showed that absorption was variable and time dependent and not determined exclusively by hydrophobicity as claimed previously. We demonstrated that two commercially available lipophilic coatings and the presence of cells affected absorption. The use of lipophilic coatings may be useful in preventing small molecule absorption by PDMS.",
keywords = "METIS-321052, IR-103086",
author = "{van Meer}, B.J. and {de Vries}, H. and K.S.A. Firth and {van Weerd}, Jasper and L.G.J. Tertoolen and H.B.J. Karperien and Pascal Jonkheijm and C. Denning and A.P. IJzerman and C.L. Mummery",
year = "2017",
doi = "10.1016/j.bbrc.2016.11.062",
language = "English",
volume = "482",
pages = "323--328",
journal = "Biochemical and biophysical research communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

Small molecule absorption by PDMS in the context of drug response bioassays. / van Meer, B.J.; de Vries, H.; Firth, K.S.A.; van Weerd, Jasper ; Tertoolen, L.G.J.; Karperien, H.B.J.; Jonkheijm, Pascal ; Denning, C.; IJzerman, A.P.; Mummery, C.L.

In: Biochemical and biophysical research communications, Vol. 482, No. 2, 2017, p. 323-328.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Small molecule absorption by PDMS in the context of drug response bioassays

AU - van Meer, B.J.

AU - de Vries, H.

AU - Firth, K.S.A.

AU - van Weerd, Jasper

AU - Tertoolen, L.G.J.

AU - Karperien, H.B.J.

AU - Jonkheijm, Pascal

AU - Denning, C.

AU - IJzerman, A.P.

AU - Mummery, C.L.

PY - 2017

Y1 - 2017

N2 - The polymer polydimethylsiloxane (PDMS) is widely used to build microfluidic devices compatible with cell culture. Whilst convenient in manufacture, PDMS has the disadvantage that it can absorb small molecules such as drugs. In microfluidic devices like "Organs-on-Chip", designed to examine cell behavior and test the effects of drugs, this might impact drug bioavailability. Here we developed an assay to compare the absorption of a test set of four cardiac drugs by PDMS based on measuring the residual non-absorbed compound by High Pressure Liquid Chromatography (HPLC). We showed that absorption was variable and time dependent and not determined exclusively by hydrophobicity as claimed previously. We demonstrated that two commercially available lipophilic coatings and the presence of cells affected absorption. The use of lipophilic coatings may be useful in preventing small molecule absorption by PDMS.

AB - The polymer polydimethylsiloxane (PDMS) is widely used to build microfluidic devices compatible with cell culture. Whilst convenient in manufacture, PDMS has the disadvantage that it can absorb small molecules such as drugs. In microfluidic devices like "Organs-on-Chip", designed to examine cell behavior and test the effects of drugs, this might impact drug bioavailability. Here we developed an assay to compare the absorption of a test set of four cardiac drugs by PDMS based on measuring the residual non-absorbed compound by High Pressure Liquid Chromatography (HPLC). We showed that absorption was variable and time dependent and not determined exclusively by hydrophobicity as claimed previously. We demonstrated that two commercially available lipophilic coatings and the presence of cells affected absorption. The use of lipophilic coatings may be useful in preventing small molecule absorption by PDMS.

KW - METIS-321052

KW - IR-103086

U2 - 10.1016/j.bbrc.2016.11.062

DO - 10.1016/j.bbrc.2016.11.062

M3 - Article

VL - 482

SP - 323

EP - 328

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

SN - 0006-291X

IS - 2

ER -