Small volume hypermethylated DNA enrichment for epigenetics

Arpita De, Wouter Sparreboom, Lennart de Vreede, L. de Vreede, Edwin Carlen, Albert van den Berg

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    Abstract

    We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.
    Original languageUndefined
    Title of host publicationThe 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012
    EditorsT. Fujii, A. Hibara, S. Takeuchi, T. Fukuba
    PublisherChemical and Biological Micro Systems Society
    Pages461-463
    Number of pages3
    ISBN (Print)1556-5904
    Publication statusPublished - 28 Oct 2012
    Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan
    Duration: 28 Oct 20121 Nov 2012
    Conference number: 16

    Publication series

    Name
    PublisherChemical and Biological Microsystems Society
    ISSN (Print)1556-5904

    Conference

    Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
    Abbreviated titleMicroTAS
    CountryJapan
    CityOkinawa
    Period28/10/121/11/12

    Keywords

    • EWI-22717
    • METIS-293257
    • IR-83540

    Cite this

    De, A., Sparreboom, W., de Vreede, L., de Vreede, L., Carlen, E., & van den Berg, A. (2012). Small volume hypermethylated DNA enrichment for epigenetics. In T. Fujii, A. Hibara, S. Takeuchi, & T. Fukuba (Eds.), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012 (pp. 461-463). Chemical and Biological Micro Systems Society.