Small volume hypermethylated DNA enrichment for epigenetics

Arpita De, Wouter Sparreboom, Lennart de Vreede, L. de Vreede, Edwin Carlen, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.
Original languageUndefined
Title of host publicationThe 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012
EditorsT. Fujii, A. Hibara, S. Takeuchi, T. Fukuba
PublisherChemical and Biological Micro Systems Society
Pages461-463
Number of pages3
ISBN (Print)1556-5904
Publication statusPublished - 28 Oct 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012
Conference number: 16

Publication series

Name
PublisherChemical and Biological Microsystems Society
ISSN (Print)1556-5904

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
Abbreviated titleMicroTAS
CountryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • EWI-22717
  • METIS-293257
  • IR-83540

Cite this

De, A., Sparreboom, W., de Vreede, L., de Vreede, L., Carlen, E., & van den Berg, A. (2012). Small volume hypermethylated DNA enrichment for epigenetics. In T. Fujii, A. Hibara, S. Takeuchi, & T. Fukuba (Eds.), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012 (pp. 461-463). Chemical and Biological Micro Systems Society.
De, Arpita ; Sparreboom, Wouter ; de Vreede, Lennart ; de Vreede, L. ; Carlen, Edwin ; van den Berg, Albert. / Small volume hypermethylated DNA enrichment for epigenetics. The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. editor / T. Fujii ; A. Hibara ; S. Takeuchi ; T. Fukuba. Chemical and Biological Micro Systems Society, 2012. pp. 461-463
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title = "Small volume hypermethylated DNA enrichment for epigenetics",
abstract = "We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84{\%} from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.",
keywords = "EWI-22717, METIS-293257, IR-83540",
author = "Arpita De and Wouter Sparreboom and {de Vreede}, Lennart and {de Vreede}, L. and Edwin Carlen and {van den Berg}, Albert",
year = "2012",
month = "10",
day = "28",
language = "Undefined",
isbn = "1556-5904",
publisher = "Chemical and Biological Micro Systems Society",
pages = "461--463",
editor = "T. Fujii and A. Hibara and S. Takeuchi and T. Fukuba",
booktitle = "The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012",

}

De, A, Sparreboom, W, de Vreede, L, de Vreede, L, Carlen, E & van den Berg, A 2012, Small volume hypermethylated DNA enrichment for epigenetics. in T Fujii, A Hibara, S Takeuchi & T Fukuba (eds), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. Chemical and Biological Micro Systems Society, pp. 461-463, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012, Okinawa, Japan, 28/10/12.

Small volume hypermethylated DNA enrichment for epigenetics. / De, Arpita; Sparreboom, Wouter; de Vreede, Lennart; de Vreede, L.; Carlen, Edwin; van den Berg, Albert.

The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. ed. / T. Fujii; A. Hibara; S. Takeuchi; T. Fukuba. Chemical and Biological Micro Systems Society, 2012. p. 461-463.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Small volume hypermethylated DNA enrichment for epigenetics

AU - De, Arpita

AU - Sparreboom, Wouter

AU - de Vreede, Lennart

AU - de Vreede, L.

AU - Carlen, Edwin

AU - van den Berg, Albert

PY - 2012/10/28

Y1 - 2012/10/28

N2 - We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.

AB - We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.

KW - EWI-22717

KW - METIS-293257

KW - IR-83540

M3 - Conference contribution

SN - 1556-5904

SP - 461

EP - 463

BT - The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012

A2 - Fujii, T.

A2 - Hibara, A.

A2 - Takeuchi, S.

A2 - Fukuba, T.

PB - Chemical and Biological Micro Systems Society

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De A, Sparreboom W, de Vreede L, de Vreede L, Carlen E, van den Berg A. Small volume hypermethylated DNA enrichment for epigenetics. In Fujii T, Hibara A, Takeuchi S, Fukuba T, editors, The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. Chemical and Biological Micro Systems Society. 2012. p. 461-463