Small volume hypermethylated DNA enrichment for epigenetics

Arpita De; Wouter Sparreboom; Lennart de Vreede; L. de Vreede; Edwin Carlen; Albert van den Berg

Small volume hypermethylated DNA enrichment for epigenetics

Arpita De, Wouter Sparreboom, Lennart de Vreede, L. de Vreede, Edwin Carlen, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Downloads (Pure)

Abstract

We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.

Original language	Undefined
Title of host publication	The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012
Editors	T. Fujii, A. Hibara, S. Takeuchi, T. Fukuba
Publisher	The Chemical and Biological Microsystems Society
Pages	461-463
Number of pages	3
ISBN (Print)	1556-5904
Publication status	Published - 28 Oct 2012
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012 Conference number: 16

Publication series

Name
Publisher	Chemical and Biological Microsystems Society
ISSN (Print)	1556-5904

Conference

Conference	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
Abbreviated title	MicroTAS
Country/Territory	Japan
City	Okinawa
Period	28/10/12 → 1/11/12

Keywords

EWI-22717
METIS-293257
IR-83540

Access to Document

http://eprints.eemcs.utwente.nl/secure2/22717/01/M.2.62.pdf

Cite this

De, A., Sparreboom, W., de Vreede, L., de Vreede, L., Carlen, E., & van den Berg, A. (2012). Small volume hypermethylated DNA enrichment for epigenetics. In T. Fujii, A. Hibara, S. Takeuchi, & T. Fukuba (Eds.), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012 (pp. 461-463). The Chemical and Biological Microsystems Society. http://eprints.eemcs.utwente.nl/secure2/22717/01/M.2.62.pdf

@inproceedings{eead632459884436bda0cce6e78f4276,

title = "Small volume hypermethylated DNA enrichment for epigenetics",

abstract = "We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.",

keywords = "EWI-22717, METIS-293257, IR-83540",

author = "Arpita De and Wouter Sparreboom and {de Vreede}, Lennart and {de Vreede}, L. and Edwin Carlen and {van den Berg}, Albert",

year = "2012",

month = oct,

day = "28",

language = "Undefined",

isbn = "1556-5904",

publisher = "The Chemical and Biological Microsystems Society",

pages = "461--463",

editor = "T. Fujii and A. Hibara and S. Takeuchi and T. Fukuba",

booktitle = "The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012",

note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",

}

De, A, Sparreboom, W, de Vreede, L, de Vreede, L, Carlen, E & van den Berg, A 2012, Small volume hypermethylated DNA enrichment for epigenetics. in T Fujii, A Hibara, S Takeuchi & T Fukuba (eds), The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. The Chemical and Biological Microsystems Society, pp. 461-463, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012, Okinawa, Japan, 28/10/12. <http://eprints.eemcs.utwente.nl/secure2/22717/01/M.2.62.pdf>

Small volume hypermethylated DNA enrichment for epigenetics. / De, Arpita; Sparreboom, Wouter; de Vreede, Lennart et al.
The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012. ed. / T. Fujii; A. Hibara; S. Takeuchi; T. Fukuba. The Chemical and Biological Microsystems Society, 2012. p. 461-463.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Small volume hypermethylated DNA enrichment for epigenetics

AU - De, Arpita

AU - Sparreboom, Wouter

AU - de Vreede, Lennart

AU - de Vreede, L.

AU - Carlen, Edwin

AU - van den Berg, Albert

N1 - Conference code: 16

PY - 2012/10/28

Y1 - 2012/10/28

N2 - We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.

AB - We present a small volume microfluidic chip for low concentration capture and elution of hypermethylated DNA (hmDNA) using solid-phase extraction. All steps of the assay have been qualitatively characterized using a real-time surface plasmon resoncance bionsensor. A caputre/elution effiency of 84% from the assay has been quantitatively characterized using calibrated fluorescence spectroscopy.

KW - EWI-22717

KW - METIS-293257

KW - IR-83540

M3 - Conference contribution

SN - 1556-5904

SP - 461

EP - 463

BT - The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, microTAS 2012

A2 - Fujii, T.

A2 - Hibara, A.

A2 - Takeuchi, S.

A2 - Fukuba, T.

PB - The Chemical and Biological Microsystems Society

T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012

Y2 - 28 October 2012 through 1 November 2012

ER -