Smart polymer brushes in nanopores: towards controlled molecular transport through pore-spanning biomembranes

G.W. de Groot

Research output: ThesisPhD Thesis - Research UT, graduation UT

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Abstract

The aim of this thesis was the development of stimulus responsive polymer brush structures as micropore switches for molecular screening. This was achieved by grafting pH responsive poly(methacrylic acid) (PMAA) brushes from nanoporous platforms that were then integrated in functional assays for membrane proteins. Several properties of the smart PMAA brush contributed to the functionality of such protein assays. Controlled transport of ions and molecules through nanopores, support of pore spanning artificial lipid bilayers and positioning of membrane proteins over the pores were achieved with the explored PMAA brush functionalized platforms.
Original languageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Vancso, Gyula J., Supervisor
  • Santonicola, M., Advisor
Award date12 Dec 2013
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-0772-1
DOIs
Publication statusPublished - 12 Dec 2013

Fingerprint

Nanopores
Brushes
Polymers
Assays
Membrane Proteins
Lipid bilayers
Screening
Switches
Ions
Molecules
methacrylic acid
Proteins

Keywords

  • METIS-299294
  • IR-88056

Cite this

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Smart polymer brushes in nanopores: towards controlled molecular transport through pore-spanning biomembranes. / de Groot, G.W.

Enschede : Ipskamp Printing, 2013. 125 p.

Research output: ThesisPhD Thesis - Research UT, graduation UT

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N2 - The aim of this thesis was the development of stimulus responsive polymer brush structures as micropore switches for molecular screening. This was achieved by grafting pH responsive poly(methacrylic acid) (PMAA) brushes from nanoporous platforms that were then integrated in functional assays for membrane proteins. Several properties of the smart PMAA brush contributed to the functionality of such protein assays. Controlled transport of ions and molecules through nanopores, support of pore spanning artificial lipid bilayers and positioning of membrane proteins over the pores were achieved with the explored PMAA brush functionalized platforms.

AB - The aim of this thesis was the development of stimulus responsive polymer brush structures as micropore switches for molecular screening. This was achieved by grafting pH responsive poly(methacrylic acid) (PMAA) brushes from nanoporous platforms that were then integrated in functional assays for membrane proteins. Several properties of the smart PMAA brush contributed to the functionality of such protein assays. Controlled transport of ions and molecules through nanopores, support of pore spanning artificial lipid bilayers and positioning of membrane proteins over the pores were achieved with the explored PMAA brush functionalized platforms.

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