Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 % cells are affected by the bubble.
Original languageUndefined
Title of host publicationEleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference
EditorsJ.L. Viovy, P Tabeling, S. Descroix, L. Malaquin
Place of PublicationSan Diego
PublisherChemical and Biological Micro Systems Society
Pages206-209
Number of pages4
ISBN (Print)978-0-9798064-0-7
Publication statusPublished - 7 Oct 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007 - Paris, France
Duration: 7 Oct 200711 Oct 2007
Conference number: 11

Publication series

NameMicro Total Analysis Systems
PublisherChemical and Biological Microsystems Society
Number7
Volume1

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007
Abbreviated titleMicroTAS
CountryFrance
CityParis
Period7/10/0711/10/07

Keywords

  • IR-62036
  • METIS-245817
  • EWI-11485

Cite this

le Gac, S., van den Berg, A., & Ohl, C. D. (2007). Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. In J. L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference (pp. 206-209). (Micro Total Analysis Systems; Vol. 1, No. 7). San Diego: Chemical and Biological Micro Systems Society.
le Gac, Severine ; van den Berg, Albert ; Ohl, C.D. / Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. editor / J.L. Viovy ; P Tabeling ; S. Descroix ; L. Malaquin. San Diego : Chemical and Biological Micro Systems Society, 2007. pp. 206-209 (Micro Total Analysis Systems; 7).
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title = "Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble.",
abstract = "We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 {\%} cells are affected by the bubble.",
keywords = "IR-62036, METIS-245817, EWI-11485",
author = "{le Gac}, Severine and {van den Berg}, Albert and C.D. Ohl",
year = "2007",
month = "10",
day = "7",
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isbn = "978-0-9798064-0-7",
series = "Micro Total Analysis Systems",
publisher = "Chemical and Biological Micro Systems Society",
number = "7",
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editor = "J.L. Viovy and P Tabeling and S. Descroix and L. Malaquin",
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}

le Gac, S, van den Berg, A & Ohl, CD 2007, Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. in JL Viovy, P Tabeling, S Descroix & L Malaquin (eds), Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. Micro Total Analysis Systems, no. 7, vol. 1, Chemical and Biological Micro Systems Society, San Diego, pp. 206-209, 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007, Paris, France, 7/10/07.

Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. / le Gac, Severine; van den Berg, Albert; Ohl, C.D.

Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. ed. / J.L. Viovy; P Tabeling; S. Descroix; L. Malaquin. San Diego : Chemical and Biological Micro Systems Society, 2007. p. 206-209 (Micro Total Analysis Systems; Vol. 1, No. 7).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble.

AU - le Gac, Severine

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AU - Ohl, C.D.

PY - 2007/10/7

Y1 - 2007/10/7

N2 - We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 % cells are affected by the bubble.

AB - We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 % cells are affected by the bubble.

KW - IR-62036

KW - METIS-245817

KW - EWI-11485

M3 - Conference contribution

SN - 978-0-9798064-0-7

T3 - Micro Total Analysis Systems

SP - 206

EP - 209

BT - Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference

A2 - Viovy, J.L.

A2 - Tabeling, P

A2 - Descroix, S.

A2 - Malaquin, L.

PB - Chemical and Biological Micro Systems Society

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ER -

le Gac S, van den Berg A, Ohl CD. Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. In Viovy JL, Tabeling P, Descroix S, Malaquin L, editors, Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. San Diego: Chemical and Biological Micro Systems Society. 2007. p. 206-209. (Micro Total Analysis Systems; 7).