Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble.

Severine le Gac; Albert van den Berg; C.D. Ohl

Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble.

Physics of Fluids

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 % cells are affected by the bubble.

Original language	Undefined
Title of host publication	Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference
Editors	J.L. Viovy, P Tabeling, S. Descroix, L. Malaquin
Place of Publication	San Diego
Publisher	The Chemical and Biological Microsystems Society
Pages	206-209
Number of pages	4
ISBN (Print)	978-0-9798064-0-7
Publication status	Published - 7 Oct 2007
Event	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007 - Paris, France Duration: 7 Oct 2007 → 11 Oct 2007 Conference number: 11

Publication series

Name	Micro Total Analysis Systems
Publisher	Chemical and Biological Microsystems Society
Number	7
Volume	1

Conference

Conference	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007
Abbreviated title	MicroTAS
Country/Territory	France
City	Paris
Period	7/10/07 → 11/10/07

Keywords

IR-62036
METIS-245817
EWI-11485

Access to Document

http://eprints.eemcs.utwente.nl/secure2/11485/01/LeGac0752.pdf

Cite this

le Gac, S., van den Berg, A., & Ohl, C. D. (2007). Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. In J. L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference (pp. 206-209). (Micro Total Analysis Systems; Vol. 1, No. 7). The Chemical and Biological Microsystems Society. http://eprints.eemcs.utwente.nl/secure2/11485/01/LeGac0752.pdf

le Gac, Severine ; van den Berg, Albert ; Ohl, C.D. / Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. editor / J.L. Viovy ; P Tabeling ; S. Descroix ; L. Malaquin. San Diego : The Chemical and Biological Microsystems Society, 2007. pp. 206-209 (Micro Total Analysis Systems; 7).

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title = "Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble.",

abstract = "We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 \% cells are affected by the bubble.",

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author = "\{le Gac\}, Severine and \{van den Berg\}, Albert and C.D. Ohl",

year = "2007",

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day = "7",

language = "Undefined",

isbn = "978-0-9798064-0-7",

series = "Micro Total Analysis Systems",

publisher = "The Chemical and Biological Microsystems Society",

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pages = "206--209",

editor = "J.L. Viovy and P Tabeling and S. Descroix and L. Malaquin",

booktitle = "Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference",

note = "11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007 ; Conference date: 07-10-2007 Through 11-10-2007",

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le Gac, S , van den Berg, A & Ohl, CD 2007, Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. in JL Viovy, P Tabeling, S Descroix & L Malaquin (eds), Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. Micro Total Analysis Systems, no. 7, vol. 1, The Chemical and Biological Microsystems Society, San Diego, pp. 206-209, 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007, Paris, France, 7/10/07. <http://eprints.eemcs.utwente.nl/secure2/11485/01/LeGac0752.pdf>

Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. / le Gac, Severine ; van den Berg, Albert; Ohl, C.D.
Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. ed. / J.L. Viovy; P Tabeling; S. Descroix; L. Malaquin. San Diego: The Chemical and Biological Microsystems Society, 2007. p. 206-209 (Micro Total Analysis Systems; Vol. 1, No. 7).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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T1 - Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble.

AU - le Gac, Severine

AU - van den Berg, Albert

AU - Ohl, C.D.

N1 - Conference code: 11

PY - 2007/10/7

Y1 - 2007/10/7

N2 - We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 % cells are affected by the bubble.

AB - We report here on both viable and permanent poration of (suspension) cells by combining microfluidics and cavitation bubbles. Cells are placed in a PDMS microchamber and exposed to a single 100-μm sized cavitation bubble. The expansion and collapse of the bubble results in shear on cells, and eventually thereby in cellular poration. We demonstrate that cell poration depends on the initial cell-bubble distance and we determine a critical distance below which > 50 % cells are affected by the bubble.

KW - IR-62036

KW - METIS-245817

KW - EWI-11485

M3 - Conference contribution

SN - 978-0-9798064-0-7

T3 - Micro Total Analysis Systems

SP - 206

EP - 209

BT - Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference

A2 - Viovy, J.L.

A2 - Tabeling, P

A2 - Descroix, S.

A2 - Malaquin, L.

PB - The Chemical and Biological Microsystems Society

CY - San Diego

T2 - 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007

Y2 - 7 October 2007 through 11 October 2007

ER -

le Gac S , van den Berg A, Ohl CD. Sonoporation of suspension cells in a microfluidic format by use of a single cavitation bubble. In Viovy JL, Tabeling P, Descroix S, Malaquin L, editors, Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference. San Diego: The Chemical and Biological Microsystems Society. 2007. p. 206-209. (Micro Total Analysis Systems; 7).