Sonoporation of suspension cells with a single cavitation bubble in a microfluidic confinement

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We report here the sonoporation of HL60 (human promyelocytic leukemia) suspension cells in a microfluidic confinement using a single laser-induced cavitation bubble. Cavitation bubbles can induce membrane poration of cells located in their close vicinity. Membrane integrity of suspension cells placed in a microfluidic chamber is probed through either the calcein release out of calcein-loaded cells or the uptake of trypan blue. Cells that are located farther away than four times R-max (maximum bubble radius) from the cavitation bubble center remain fully unaffected, while cells closer than 0.75 R-max become porated with a probability of >75%. These results enable us to define a distance of 0.75 R-max as a critical interaction distance of the cavitation bubble with HL60 suspension cells. These experiments suggest that flow-induced poration of suspension cells is applicable in lab-on-a-chip systems, and this might be an interesting alternative to electroporation.
Original languageUndefined
Article number10.1039/b712897p
Pages (from-to)1666-1672
Number of pages7
JournalLab on a chip
Issue number7/12
Publication statusPublished - 2007


  • EWI-11700
  • IR-62103
  • METIS-245924

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