Sorting catalytically active polymersomes by flow cytometry

Madhavan Nallani, Rob Woestenenk, Hans-Peter de Hoog, Stijn F.M. van Dongen, Jan Boezeman, Jeroen Johannes Lambertus Maria Cornelissen, Roeland J.M. Nolte, Jan C.M. van Hest

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Enzyme-filled stable polymeric capsules (polymersomes) that are permeable to low-molecular-weight (pro-fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build-up of fluorescent product that is prevented from leaking out by co-encapsulation of a trapping agent.
Original languageUndefined
Pages (from-to)1138-1143
Number of pages6
JournalSmall
Volume5
Issue number10
DOIs
Publication statusPublished - 2009

Keywords

  • METIS-263216
  • IR-100019

Cite this

Nallani, M., Woestenenk, R., de Hoog, H-P., van Dongen, S. F. M., Boezeman, J., Cornelissen, J. J. L. M., ... van Hest, J. C. M. (2009). Sorting catalytically active polymersomes by flow cytometry. Small, 5(10), 1138-1143. https://doi.org/10.1002/smll.200801204