Sorting catalytically active polymersomes by flow cytometry

Madhavan Nallani; Rob Woestenenk; Hans-Peter de Hoog; Stijn F.M. van Dongen; Jan Boezeman; Jeroen Johannes Lambertus Maria Cornelissen; Roeland J.M. Nolte; Jan C.M. van Hest

doi:10.1002/smll.200801204

Sorting catalytically active polymersomes by flow cytometry

Madhavan Nallani, Rob Woestenenk, Hans-Peter de Hoog, Stijn F.M. van Dongen, Jan Boezeman, Jeroen Johannes Lambertus Maria Cornelissen, Roeland J.M. Nolte, Jan C.M. van Hest

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Abstract

Enzyme-filled stable polymeric capsules (polymersomes) that are permeable to low-molecular-weight (pro-fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build-up of fluorescent product that is prevented from leaking out by co-encapsulation of a trapping agent.

Original language	Undefined
Pages (from-to)	1138-1143
Number of pages	6
Journal	Small
Volume	5
Issue number	10
DOIs	https://doi.org/10.1002/smll.200801204
Publication status	Published - 2009

Keywords

METIS-263216
IR-100019

Access to Document

10.1002/smll.200801204

Cite this

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title = "Sorting catalytically active polymersomes by flow cytometry",

abstract = "Enzyme-filled stable polymeric capsules (polymersomes) that are permeable to low-molecular-weight (pro-fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build-up of fluorescent product that is prevented from leaking out by co-encapsulation of a trapping agent.",

keywords = "METIS-263216, IR-100019",

author = "Madhavan Nallani and Rob Woestenenk and {de Hoog}, Hans-Peter and {van Dongen}, {Stijn F.M.} and Jan Boezeman and Cornelissen, {Jeroen Johannes Lambertus Maria} and Nolte, {Roeland J.M.} and {van Hest}, {Jan C.M.}",

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T1 - Sorting catalytically active polymersomes by flow cytometry

AU - Nallani, Madhavan

AU - Woestenenk, Rob

AU - de Hoog, Hans-Peter

AU - van Dongen, Stijn F.M.

AU - Boezeman, Jan

AU - Cornelissen, Jeroen Johannes Lambertus Maria

AU - Nolte, Roeland J.M.

AU - van Hest, Jan C.M.

PY - 2009

Y1 - 2009

N2 - Enzyme-filled stable polymeric capsules (polymersomes) that are permeable to low-molecular-weight (pro-fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build-up of fluorescent product that is prevented from leaking out by co-encapsulation of a trapping agent.

AB - Enzyme-filled stable polymeric capsules (polymersomes) that are permeable to low-molecular-weight (pro-fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build-up of fluorescent product that is prevented from leaking out by co-encapsulation of a trapping agent.

KW - METIS-263216

KW - IR-100019

U2 - 10.1002/smll.200801204

DO - 10.1002/smll.200801204

M3 - Article

SN - 1613-6810

VL - 5

SP - 1138

EP - 1143

JO - Small

JF - Small

IS - 10

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