Speckle correlation resolution enhancement of wide-field fluorescence imaging

H. Yilmaz, E.G. van Putten, J. Bertolotti, Aart Lagendijk, Willem L. Vos, Allard Mosk

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Abstract

High-resolution fluorescence imaging is essential in nanoscience and biological sciences. Due to the diffraction limit, conventional imaging systems can only resolve structures larger than 200 nm. Here, we introduce a new fluorescence imaging method that enhances the resolution by using a high-index scattering medium as an imaging lens. Simultaneously, we achieve a wide field of view. We develop a new image reconstruction algorithm that converges even for complex object structures. We collect two-dimensional fluorescence images of a collection of 100 nm diameter dye-doped nanospheres, and demonstrate a deconvolved Abbe resolution of 116 nm with a field of view of 10 μm×10  μm . Our method is robust against optical aberrations and stage drifts, and therefore is well suited to image nanostructures with high resolution under ambient conditions.
Original languageEnglish
Pages (from-to)424-429
JournalOptica
Volume2
Issue number5
DOIs
Publication statusPublished - 27 Apr 2015

Keywords

  • METIS-310342
  • IR-95671

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